Effectiveness of noninvasive embryo chromosome screening in preimplantation genetic testing at different ages
10.3760/cma.j.cn431274-20210803-00842
- VernacularTitle:无创胚胎染色体筛查在不同年龄段胚胎植入前遗传学检测的有效性
- Author:
Bolan SUN
1
;
Yong WANG
;
Chunhui ZHANG
;
Xiangli ZOU
;
Sixi WEN
;
Liang ZHOU
;
Weiping QIAN
Author Information
1. 北京大学深圳医院生殖医学中心，广东省生殖与遗传重点实验室 518000
- Keywords:
Embryo implantation;
Blastula;
Preimplantation diagnosis;
Noninvasive chromosome screening
- From:
Journal of Chinese Physician
2021;23(9):1290-1293,1298
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effectiveness of noninvasive embryo chromosome screening (NICS) based on blastocyst culture medium and cystic fluid in preimplantation genetic detection (PGT) of embryos in different age groups.Methods:A retrospective analysis of 62 couples who underwent PGT assisted pregnancy in Shenzhen Hospital of Peking University from January 2019 to June 2021. A total of 310 blastocysts were biopsied. At the same time, D3-6 blastocyst culture medium and blastocyst cavity fluid were collected for NICS. According to the age of the patients, they were divided into three groups: <35 years old group, 35≤age<40 years old group and ≥40 years old group. The results of NICS were compared with those of embryonic trophoblast (TE) biopsy in each group, and the consistency, sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) were calculated. Then the consistency, sensitivity, specificity, positive predictive value and negative predictive value of NICS and TE among the three age groups were statistically analyzed.Results:There was no statistically significant difference in the consistency rate of NICS and TE among the three age groups ( P>0.05), but there was an upward trend in the elderly group (35≤age<40 years and ≥40 years). There was no statistically significant difference in specificity, sensitivity and PPV among the three age groups ( P>0.05). There was significant difference in NPV between the ≥40 years group and the other two groups ( P<0.05). Conclusions:There was no statistical difference in the effectiveness of NICS among different age groups, However, there was an increasing trend in people ≥35 years of age.
