Study on the cisplatin-sensitization effect of tripterygium glycosides on cisplatin-resistant human epithelial ovarian cancer cells (SKOV3/DDP) in vitro
- VernacularTitle:雷公藤多苷增强耐顺铂人上皮性卵巢癌SKOV3/DDP细胞株顺铂敏感性的体外研究
- Author:
Wencheng LIU
1
;
Buzhen TAN
;
Xinlu ZHAN
;
Hui HU
;
Genhua HUANG
;
Qing CAO
Author Information
1. 南昌大学第二附属医院,妇产科,南昌330006,江西
- Keywords:
tripterygium glycosides;
SKOV3/DDP;
cisplatin;
cisplatin-sensitization effect;
STAT3
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2017;22(12):1364-1370
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To study the cisplatin-sensitization effect and mechanism of tripterygium glycosides on cisplatin-resistant human epithelial ovarian cancer cells (SKOV3/DDP).METHODS:The SKOV3/DDP cells in exponential phase of growth were randomly divided into eight groups:blank control group,10 μg/mL DDP group,50 μg/mL GTW group,800 μg/mL GTW group,3 200 μg/mL GTW group,10 μg/mL DDP + 50 μg/mL GTW group,10 μg/mL DDP + 800 μg/mL GTW group and 10 μg/mL DDP + 3 200 μg/mL GTW group.Cell counting kit 8 and flow cytometry and western blot were used to detect the growth inhibiting rate and apoptosis rate and relative expression of GST-π,MDR1,STAT3,p-STAT3 of SKOV3/DDP cells of every group.RESULTS:DDP and GTW produce an additive effect when used concurrently in inhibiting growth of SKOV3/DDP cells.800 μg/mL GTW or 3 200 μg/mL GTW combined with 10 μg/mL DDP can significantly induce apoptosis of SKOV3/DDP cells and downregulate the expression of p-STAT3 (P ＜ 0.05).CONCLUSION:GTW can significantly enhance sensitivity of SKOV3/DDP cells to DDP.The underlying mechanism may be related with down-regulating the expression of p-STAT3 in STAT3 pathway.
