Effects of Huashenzilu on chronic eczema in mice model
- VernacularTitle:花参子露对小鼠慢性湿疹模型的疗效研究
- Author:
Dandan YAN
1
;
Fang HUANG
;
Lihua XU
Author Information
1. 中国药科大学,南京211100,江苏
- Keywords:
Huashenzilu;
chronic eczema;
immune response
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2017;22(12):1358-1363
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe clinical the effects of Huashenzilu on mice models with acute and chronic eczema.METHODS:Eczema was induced by repeated local exposure to DNCB,and then the mice received Huashenzilu.Using ELISA kit method to test the serum concentration of IL-2,IL-4,TNF-α,IFN-γ,and HE staining to test histopathological changes.The ear swelling and scratching index were measured,and ear allergy score were measured according to the standard for evaluation.RESULTS:After the treatment of Huashenzilu,the serum concentration of IL-4,TNF-α significantly increased and the serum concentration of IL-2,IFN-γ significantly decreased in the chronic mice compared to the model group (P ＜ 0.01).The ear swelling,scratching index and ear allergy score were significantly decreased.The phenomenon of epidermal thickening,acanthosis layer cavitation,and mast cells,macrophages infiltration were improved compared to the model group.CONCLUSION:Huashenzilu can control the chronic eczema in mice through the mechanism of regulating the balance between Th1 and Th2 immune response.
