Effects of dexmedetomidine on JNK in A549 cells with hypoxia/reoxygenation injury
- VernacularTitle:右美托咪定对A549细胞缺氧/复氧损伤时JNK的影响
- Author:
Ziyin LUO
1
,
2
;
Bingqian XIANG
;
Hui GAO
;
Xiaoxiao QIU
;
Xiaoying QIAN
;
Wantie WANG
Author Information
1. 温州医科大学缺血-再灌注损伤研究所,温州325035,浙江
2. 湖北省中西医结合医院病理科,武汉430015,湖北
- Keywords:
JNK;
dexmedetomidine;
hypoxia/reoxygenation injury;
A549 cells;
cell apoptosis
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2017;22(12):1346-1351
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effects and c-Jun N-terminal kinase(JNK) expression of dexmedetomidine (DEX) on A549 cells with hypoxia/reoxygenation (H/R) injury.METHODS:A549 cells were cultivated and were randomly divided into four groups (n =10):control group (N),DEX group (D),hypoxia/reoxygenation injury group (H),hypoxia/reoxygenation injury + DEX interfere group (HD).After all models were completed,the morphological changes of A549 cells were observed under the inverted microscope.Cell activity was detected by CCK-8 and the apoptosis index (AI) was detected by in situ end labeling (TUNEL) method.The expression of GRP78,p-JNK,caspase-3 at protein levels and GRP78,JNK mRNA were detected by Western blot and RT-PCR.RESULTS:Compared with N group,the number of adherent cells in H group decreased significantly,and cell morphology changed.The expression of OD value in H group decreased obviously (P ＜ 0.01),the expression of AI value,GRP78,p-JNK,caspase-3 protein and GRP78,JNK mRNA were significantly increased (P＜ 0.01).HD group compared with H group,the cell damage alleviated,the expression of OD value was increased (P ＜ 0.01),the number of apoptosis cells and the AI value in HD group were significantly decreased (P ＜ 0.01).Dramatically decreased the expression of p-JNK,caspase-3 protein and JNK mRNA (P ＜ 0.01).CONCLUSION:DEX can effectively alleviate A549 cells damage induced by H/R injury,which may be related to inhibition of the JNK pathway.
