Experimental study of benazepril intervene Wnt signaling inhibits renal fibrosis in UUO rats
- VernacularTitle:苯那普利干预Wnt信号通路抑制UUO大鼠肾纤维化的实验研究
- Author:
Yanyun ZHAO
1
;
Xiaoyan WU
;
Guomin WANG
;
Yulin FENG
;
Feixia DONG
Author Information
1. 浙江中医药大学附属温州中医院药剂科,温州325000,浙江
- Keywords:
benazepril;
renal interstitial fibrosis;
unilateral ureteral obstruction;
Wnt signaling
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2017;22(12):1332-1339
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the antagonism mechanism of benazepril on renal fibrosis of unilateral uretreal obstructire (UUO).METHODS:Fiftyfour Sprague-Dawley (SD) rats were randomly divided into three groups:sham operation group,model group,benazepril group.All the other groups were treated with surgery method of unilateral ureteral ligation to establish a rat model of renal fibrosis except the sham operation group.The pathological changes were observed by Masson staining;the positive staining expression of TGF-β1,Col-Ⅳ,Wnt1,Wnt4 in frozen sections were observed by immunofluorescence staining;the expressions TGF-β1,Col-Ⅳ,Wnt1 and Wnt4 mRNA in renal tissues were detected by real time fluorescence quantitative PCR.RESULTS:Masson staining results:compared with the sham group,the parts of blue dye collagen fiber and the degree of renal fibrosis increased significantly in all modeled groups;compared with the model group,the parts of blue dye collagen fiber and the degree of renal fibrosis decreased significantly in benazepril group.Immunofluorescence and PCR results:compared with the model group,the positive expression of Wnt1,Wnt4,TGF-β1,Col-Ⅳ and mRNA in the benazepril group decreased significantly.CONCLUSION:Benazepril can improve renal fibrosis by inhibiting the expression of TGF-β1,Col-Ⅴ,Wnt1,Wnt4 and ameliorating the level of renal fibrosis.
