Engineered polymer nanoparticles incorporating L-amino acid groups as affinity reagents for fibrinogen
- Author:
Zhu YONGYAN
1
,
2
,
3
;
Liu RUIXUAN
;
Wu DENGYU
;
Yu QIANQIAN
;
J.Shea KENNETH
;
Zhu QUANHONG
Author Information
1. School of Traditional Chinese Medicine,Southern Medical University,Guangzhou,510515,China
2. Guangdong Provincial Key Laboratory of Chinese Medicine Pharmaceutics,Guangzhou,510515,China
3. Guangdong Provincial Engineering Laboratory of Chinese Medicine Preparation Technology,Guangzhou,510515,China
- Keywords:
Synthetic polymer nanoparticles;
Amino-acid monomers;
Arginine;
Fibrinogen;
Affinity reagent;
Protein interaction
- From:
Journal of Pharmaceutical Analysis
2021;11(5):596-602
- CountryChina
- Language:Chinese
-
Abstract:
Synthetic polymer hydrogel nanoparticles(NPs)were developed to function as abiotic affinity reagents for fibrinogen.These NPs were made using both temperature-sensitive N-isopropyl acrylamide(NIPAm)and L-amino acid monomers.Five kinds of L-amino acids were acryloylated to obtain functional mono-mers:L-phenylalanine(Phe)and L-leucine(Leu)with hydrophobic side chains,L-glutamic acid(Glu)with negative charges,and L-lysine(Lys)and L-arginine(Arg)with positive charges.After incubating the NPs with fibrinogen,y-globulin,and human serum albumin(HSA)respectively,the NPs that incorporated N-acryloyl-Arg monomers(AArg@NPs)showed the strongest and most specific binding affinity to fibrin-ogen,when compared with y-globulin and HSA.Additionally,the fibrinogen-AArg binding model had the best docking scores,and this may be due to the interaction of positively charged AArg@NPs and the negatively charged fibrinogen D domain and the hydrophobic interaction between them.The specific adsorption of AArg@NPs to fibrinogen was also confirmed by the immunoprecipitation assay,as the AArg@NPs selectively trapped the fibrinogen from a human plasma protein mixture.AArg@NPs had a strong selectivity for,and specificity to,fibrinogen and may be developed as a potential human fibrinogen-specific affinity reagent.
