A DNA-based nanocarrier for efficient cancer therapy
- Author:
Abbas MUHAMMAD
1
;
Mirza Muhammad Faran Ashraf Baig
;
Zhang YALIANG
;
Yang YU-SHUN
;
Wu SONGYU
;
Hu YIQIAO
;
Wang ZHONG-CHANG
;
Zhu HAI-LIANG
Author Information
1. State Key Laboratory of Pharmaceutical Biotechnology,Nanjing University,Nanjing,210023,PR China
- Keywords:
DNA-nanothread;
Cisplatin;
Drug delivery;
Scavenger receptors;
Caveolae;
HeLa cell line
- From:
Journal of Pharmaceutical Analysis
2021;11(3):330-339
- CountryChina
- Language:Chinese
-
Abstract:
The study aimed to achieve enhanced targeted cytotoxicity and cell-internalization of cisplatin-loaded deoxyribonucleic acid-nanothread (CPT-DNA-NT),mediated by scavenger receptors into HeLa cells.DNA-NT was developed with stiff-topology utilizing circular-scaffold to encapsulate CPT.Atomic force microscopy (AFM) characterization of the DNA-NT showed uniformity in the structure with a diameter of 50-150 nm and length of 300-600 nm.The successful fabrication of the DNA-NT was confirmed through native-polyacrylamide gel electrophoresis analysis,as large the molecular-weight (polymeric) DNA-NT did not split into constituting strands under applied current and voltage.The results of cell viability confirmed that blank DNA-NT had the least cytotoxicity at the highest concentration (512 nM) with a viability of 92％ as evidence of its biocompatibility for drug delivery.MTT assay showed superior cyto-toxicity of CPT-DNA-NT than that of the free CPT due to the depot release of CPT after DNA-NT inter-nalization.The DNA-NT exhibited targeted cell internalizations with the controlled intracellular release of CPT (from DNA-NT),as illustrated in confocal images.Therefore,in vitro cytotoxicity assessment through flow cytometry showed enhanced apoptosis (72.7％) with CPT-DNA-NT (compared to free CPT;64.4％).CPT-DNA-NT,being poly-anionic,showed enhanced endocytosis via scavenger receptors.
