The study on bone marrow-derived mesenchymal stem cells in alleviating tubulointerstitial injury in unilateral ureteral obstruction model

VernacularTitle:骨髓间充质干细胞延缓单侧输尿管梗阻模型肾脏纤维化的研究
Author: Li XING ¹ ; Chengyuan YU
Author Information

1. 哈尔滨医科大学附属第一医院肾内一科　150001
Keywords: Myeloid progenitor cells; Ureteral obstruction; Unilateral; Renal fibrosis
From: Chinese Journal of Postgraduates of Medicine 2021;44(7):601-605
CountryChina
Language:Chinese
Abstract: Objective:To investigate the effect and mechanism of bone marrow-derived mesenchymal stem cells (MSCs) in alleviating renal injury in unilateral ureteral obstruction (UUO) model.Methods:MSCs were cultured, and then transplanted into a UUO model through the tail vein. Histology, cell apoptosis, peritubular capillary (PTC) loss and β-catenin expression were examined on the fourteen day after surgery.Results:Renal interstitial fibrosis in the MSCs group was significantly attenuated compared with that in the UUO group (HE: 1.60 ± 0.35 vs. 3.34 ± 0.23; MASSON: 21.32 ± 7.54 vs. 51.08 ± 4.45). Moreover, MSCs treatment inhibited the loss of peritubular capillaries (PTC) (13.56 ± 4.65 vs. 60.16 ± 10.24), cell apoptosis (14.32 ± 3.54 vs. 28.16 ± 6.21) and β-catenin expression (29.33 ± 6.45 vs. 39.51 ± 8.42).Conclusions:MSCs infusion is a promising therapeutic strategy for promoting kidney repair in chronic renal fibrosis model. The mechanism maybe that it inhibits the loss of peritubular capillaries (PTC) , cell apoptosis and β-catenin expression.