Array-based comparative genomic hybridization combined with quantitative fluorescence-polymerase chain reaction analysis of spontaneous abortion tissue
10.3760/cma.j.cn115455-20201105-01519
- VernacularTitle:微阵列比较基因组杂交联合荧光定量聚合酶链反应检测自然流产组织结果分析
- Author:
Yuan ZHANG
1
;
Jing ZHANG
;
Ling LIU
;
Xiaoxue LIU
;
Ming SHI
Author Information
1. 大连医科大学在读研究生 116044
- Keywords:
Abortion, spontaneous;
Microarray analysis;
Genome;
Abnormal Karyotype
- From:
Chinese Journal of Postgraduates of Medicine
2021;44(7):596-601
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To detect and analyze the spontaneous abortion tissue by Array-based comparative genomic hybridization (Array-CGH) combined with quantitative fluorescence polymerase chain reaction (QF-PCR), and provide genetic guidance for spontaneous abortion prevention and prenatal testing.Methods:A total of 345 patients who were diagnosed with spontaneous abortion at Dalian Women′s and Children′s Medical Center between July 2016 to September 2019 were continuously collected. Array-CGH combined with QF-PCR technology was used to analyze and collect abortion tissues that met the inclusion criteria.Results:QF-PCR detected a total of 213 cases of abnormal chromosome number, and Array-CGH supplemented the detection of 24 cases of abnormal chromosome structure. The genetic abnormality detection rate reached 68.7% (237/345) , and common abortion tissues′ chromosomal abnormalities in this area were detected. The incidence of abortion tissues′ chromosomal abnormalities in the elder pregnant women (≥ 35 years) and earlier trimester pregnant women (<10 weeks) was significantly increased: 84.43% (141/167) vs. 53.93% (96/178), 59.42% (205/284) vs. 9.28% (32/61).Conclusions:Array-CGH combined with QF-PCR is comprehensive and complementary. Some karyotype abnormalities are more common in abortion tissues. The elder and earlier trimester pregnant women should accept genetic counseling.
