The relationship between interleukin-1 system gene polymorphism and change in bone mineral density after hormone replacement therapy in postmenopausal Korean women.
- Author:
Jung Gu KIM
1
;
Byung Chul JEE
;
Seung Yup KU
;
Chang Suk SUH
;
Seok Hyun KIM
;
Young Min CHOI
;
Shin Yong MOON
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Seoul, Korea. kimjg@plaza.snu.ac.kr
- Publication Type:Original Article
- Keywords:
Postmenopausal women;
IL-1 system;
Gene polymorphism;
Bone mineral density;
Hormone therapy
- MeSH:
Absorptiometry, Photon;
Blood Cells;
Bone Density*;
Enzyme-Linked Immunosorbent Assay;
Female;
Femur;
Genotype;
Hormone Replacement Therapy*;
Humans;
Immunoassay;
Interleukin 1 Receptor Antagonist Protein;
Interleukin-1*;
Osteocalcin;
Sequence Analysis, DNA;
Spine;
Tandem Repeat Sequences
- From:Korean Journal of Obstetrics and Gynecology
2005;48(8):1950-1961
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To investigate the relationship between interleukin-1 (IL-1) system gene polymorphisms, and change in production of IL-1 by whole blood cells (WBCs), and bone mineral density (BMD) after hormone replacement therapy (HRT) in postmenopausal Korean women. METHODS: The IL-1alpha C(-889)T polymorphism, IL-1beta C(-511)T polymorphism and 86-base pair variable number tandem repeat (VNTR) polymorphism in the IL-1 receptor antagonist (IL-1ra) gene was analyzed by restriction fragment length polymorphsim (REFLP), polymerase chain reaction-Genescan, and DNA sequencing in 206 postmenopausal Korean women receiving sequential HRT for 1 year. IL-1alpha, IL-1beta, and IL-1ra produced by WBCs cultured with lipopolysaccharide for 2 days, and serum CrossLaps (CTX), and osteocalcin were measured using enzyme-linked immunosorbent assay (ELISA) and immunoassay respectively. BMD at the lumbar spine and proximal femur was determined by dual energy X-ray absorptiometry. RESULTS: The IL-1 system genotypes were not distributed differently between HRT-responders and HRT-nonresponders (women who lose more than 3% of bone mass per year), and no differences in the annual percent change of BMD after HRT were noted among IL-1 system genotypes. After HRT, the production of IL-1alpha by WBCs decreased but no changes in the production of other components of IL-1 system were noted. There were no differences in the 6 month changes of bone turnover markers and the production of IL-1 system components by WBCs and IL-1beta/IL-1ra ratio after HRT across IL-1 system genotypes. Except a negative correlation between IL-1alpha with annual change of BMD at trochanter after HRT, the production of other components of IL-1 system by WBCs and their changes after HRT of 6 month did not correlated with annual change of BMD after HRT CONCLUSION: The IL-1 system gene polymorphisms do not affect change in BMD, and change in the production of ILs by WBCs after HRT in postmenopausal Korean women.
