Clinical characteristics of 3 children with severe tuberculosis and analysis of the results of metagenomic next-generation sequencing
10.3760/cma.j.cn101070-20210520-00562
- VernacularTitle:儿童重症结核病3例临床特点及宏基因组二代测序结果分析
- Author:
Yuanyuan XU
1
;
Chang WANG
;
Wenjia TONG
;
Yuanyuan DUAN
;
You WU
;
Danqun JIN
Author Information
1. 安徽省儿童医院儿童重症监护病房，合肥 230051
- Keywords:
Metagenomic next-generation sequencing;
Tuberculosis;
Pediatric intensive care unit;
Diagnosis
- From:
Chinese Journal of Applied Clinical Pediatrics
2021;36(15):1187-1190
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the clinical characteristics of children with severe tuberculosis (TB) in pediatric intensive care unit (PICU) and the diagnostic viability of metagenomic next-generation sequencing (mNGS).Methods:The clinical data and mNGS results of 3 children with severe TB admitted to PICU of Anhui Provincial Children′s Hospital from January 2018 to December 2020 were retrospectively analyzed.The literature reporting children with TB diagnosed by applying mNGS were searched in China National Knowledge Infrastructure, Wanfang data, VIP and PubMed data bases, all of which were searched from the establishment of the database to March 2021, and the relevant literature was reviewed.Results:(1)All of 3 cases, including 2 males and 1 female, all had fever.One case was diagnosed with X-linked severe combined immunodeficiency (X-SCID). Acid-fast stain tests of body fluid, purified protein derivative (PPD) tests and T-cell spot tests of tuberculosis infection (T-SPOT.TB) were conducted in 3 cases, with the test results being negative, and the early diagnosis was confirmed with mNGS.Two cases were discharged from the hospital after being switched to special anti-TB treatment.(2)A total of 3 articles were retrieved, in which 7 children were reported.One male with X-SCID combined with bacillus Calmette-Guérin-associated hemophagocytic syndrome and 6 children with tuberculous meningitis were subject to early diagnosis with mNGS (detection of Mycobacterium tuberculosis complex).Conclusions:It is promising for the application of mNGS in rapid pathogen diagnosis of children with severe TB, especially in children with immunodeficiency.It could optimize early diagnosis and treatment to improve prognosis.
