Application of next-generation sequencing technology in pathogen detection of lower respiratory tract infection in children
10.3760/cma.j.cn101070-20191106-01099
- VernacularTitle:二代测序技术在儿童下呼吸道感染病原检测中的应用
- Author:
Mengxue CHEN
1
;
Jianhua ZHANG
Author Information
1. 上海交通大学医学院附属新华医院儿科 200092
- Keywords:
Next-generation sequencing;
Lower respiratory tract infection;
Pathogen detection;
Child
- From:
Chinese Journal of Applied Clinical Pediatrics
2021;36(14):1115-1118
- CountryChina
- Language:Chinese
-
Abstract:
Next-generation sequencing (NGS), also known as high-throughput sequencing, is more efficient compared with Sanger sequencing that has become the standard method of clinical DNA sequencing, and can obtain a large amount of information in a relatively short time at a lower cost.NGS has broad prospects in such aspects as diagnosing the pathogen of lower respiratory tract infection in children, identifying the pathogen of cross-infection in hospital, drug resistance research and vaccine development.It is still worth conducting further studies on the approach to improve the specificity and sensitivity of diagnosis and to optimize NGS.
