Analysis of serum anti-EB virus antibody in children with infectious mononucleosis
10.3760/cma.j.cn101070-20200122-00093
- VernacularTitle:传染性单核细胞增多症患儿血清抗EB病毒抗体检测结果分析
- Author:
Xiaolei GUAN
1
;
Jing YAN
;
Qiuhong ZHAO
;
Qiuping LI
;
Jianing YAO
;
Yue CUI
;
Junhong AI
;
Ran WANG
;
Zhengde XIE
Author Information
1. 国家儿童医学中心，首都医科大学附属北京儿童医院，北京市儿科研究所，儿科学国家重点学科，国家呼吸系统疾病临床医学研究中心，教育部儿科重大疾病研究重点实验室，儿童呼吸道感染性疾病研究北京市重点实验室，中国医学科学院儿童危重感染诊治创新单元 100045
- Keywords:
Epstein-Barr virus;
Infectious mononucleosis;
Antibody affinity
- From:
Chinese Journal of Applied Clinical Pediatrics
2021;36(13):1004-1006
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To understand the profile type of serum Epstein-Barr virus (EBV) antibodies in children with infectious mononucleosis (IM), and to analyze the significance of viral capsid antigen (VCA) IgG antibody affinity in the diagnosis of IM.Methods:Retrospective analysis was performed on the results of the serum anti-EBV antibody profile and plasma EBV nucleic acid test of 150 hospitalized children with IM diagnosed in Beijing Children′s Hospital, Capital Medical University, from May 2016 to May 2019.Anti-EBV antibody profiles, including anti-VCA-IgG, anti-VCA-IgM, anti-early antigen (EA) IgA, anti-EBV nuclear antigen (EBNA) IgG, and anti-VCA-IgG affinity, were detected by enzyme-linked immunosorbent assay (ELISA). Plasma EBV nucleic acids were detected by real-time quantitative PCR.Results:There were mainly two types of anti-EBV antibody profiles in 150 children with IM: (1)130 cases who were positive for anti-VCA-IgM/IgG, negative for anti-EBNA-IgG and positive for anti-VCA-IgG antibodies with low affinity, accounting for 86.7% (130/150 cases), of which 50 cases were positive for anti-early antigen IgA; (2)18 cases who were negative for anti-VCA-IgM, positive for anti-VCA-IgG, negative for anti-EBNA-IgG and positive for anti-VCA-IgG antibody with low affinity, accounting for 12.0% (18/150 cases), of which 5 cases were positive for anti-EA IgA.EBV DNA was measured in 132 children, with a posi-tive rate of 37.9% (50/132 cases).Conclusions:There were several types of serum EBV antibody profiles in children with IM, 12.0% of patients with IM in this study were negative for anti-VCA-IgM, and the diagnosis of IM was confirmed by the affinity of anti-VCA IgG.
