Effect of cabazitaxel on metastasis and proliferation of lung cancer cell A549 and its mechanism
10.3760/cma.j.cn.115807-20201013-00310-20210512
- VernacularTitle:卡巴他赛对肺癌细胞A549转移与增殖能力的影响及其作用机制
- Author:
Ling WU
1
;
Huijing FENG
;
Xiaoling YANG
;
Hongxia GUO
;
Junping ZHANG
Author Information
1. 山西白求恩医院肿瘤中心胸部肿瘤科，太原 030006
- Keywords:
Cabazitaxel;
Lung cancer;
Extracellular matrix;
Tumor metastasis
- From:
Chinese Journal of Endocrine Surgery
2021;15(3):304-308
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of cabazitaxel on lung cancer cell metastasis and proliferation and the related mechanisms.Methods:Lung cancer cells A549 were divided into two groups. The experimental group were cultured with a low concentration of 50 μg/ml cabazitaxel, and the control group were cultured with an equal volume of a solution of cabazitaxel. The proliferation ability of the two groups of cells was examined using CCK8 and plate cloning experiments. The migration ability of A459 cells was verified by transwell and cell scratch experiments. The expression levels of MMP2/9, CDK4/6, and P16 protein were detected by Western blotting.Results:Compared with the control group, the cell proliferation ability of the A549 cell was weakened in the experimental group. The plate clone formation rate of the experimental group was 17.5%±2.3%, and A549 cell clone formation rate of the control group was 74.8%±4.5%. The cloning ability was reduced in the experimental group. Western blot results showed that the expression of CDK4/6 in the experimental group was down-regulated and the expression of P16 was up-regulated. The scratch healing percentage of the cells in the experimental group was 56.2%±3.8%, and the scratch healing percentage of the cells in the control group was 86.8%±5.2%. The scratch healing ability of the cells in the experimental group decreased. The transwell results showed that the experimental group had 35±4 cells per field of view, while it was 78±9 in the control group. The cell migration ability of the experimental group was decreased. Western blot results showed that the expression of MMP2/9 in the experimental group was down-regulated.Conclusion:Cabazitaxel leads to a decrease in the metastasis ability of lung cancer cells A549 through the extracellular matrix pathway, and inhibits cell proliferation by up-regulating P16.
