Decorin and TGF-beta Expression after Partial Myotomy of the Extraocular Muscle in Rat.
10.3341/jkos.2013.54.1.143
- Author:
Young Jin LIM
1
;
Gyu Nam KIM
;
Na Eun LEE
;
Sung Jae KIM
;
Ji Myong YOO
Author Information
1. Department of Ophthalmology, Gyeongsang National University School of Medicine, Jinju, Korea. YJM@gnu.ac.kr
- Publication Type:Original Article
- Keywords:
Decorin;
Extraocular muscle;
Fibrosis;
Tumor growth factor
- MeSH:
Animals;
Conjunctiva;
Decorin*;
Fibrosis;
Immunohistochemistry;
Microscopy;
Rats*;
Rats, Sprague-Dawley;
Sutures;
Transforming Growth Factor beta*
- From:Journal of the Korean Ophthalmological Society
2013;54(1):143-148
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To report the expression of decorin and TGF-beta in partial myotomy of the extraocular muscle in rats. METHODS: Partial myotomy of the superior rectus muscle was performed on the right eye of 10 Sprague-Dawley rats followed by exposure of the left superior rectus muscle and a simple suture of the conjunctiva. The bilateral superior rectus muscle was obtained from all rats at 2 weeks postoperatively. The tissues were observed under light microscopy with hematoxylin-eosin, Masson's trichrome staining and immunohistochemistry. RESULTS: Histological examinations of the surgical area at 2 weeks after postoperatively showed irregularly concentrated fibrosis on light microscopy with hematoxylin-eosin and Masson's trichrome staining of the experimental eyes. Immnohistochemistry showed that expression of decorin was in the same location as TGF-beta in the experimental group. CONCLUSIONS: The expression of decorin was found in the healing process after partial myotomy of the extraocular muscle in rats. Immunohistochemistry showed that expression of decorin was in the same location as with TGF-beta.
