WWC2-AS1/miR-382-5p/FZD3 axis regulates proliferation and apoptosis of ovarian granulosa cells in polycystic ovary syndrome
10.3760/cma.j.cn.115807-20210105-00005
- VernacularTitle:WWC2-AS1/miR-382-5p/FZD3轴调控多囊卵巢综合征卵巢颗粒细胞增殖、凋亡
- Author:
Xueyan SUN
1
;
Liping YANG
;
Xuhong GONG
;
Zhenli SUN
Author Information
1. 山东省烟台市烟台山医院生殖医学中心 264000
- Keywords:
WWC2-AS1;
miR-382-5p;
FZD3;
Polycystic ovary syndrome;
Granulosa cell
- From:
Chinese Journal of Endocrine Surgery
2021;15(3):264-268
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of WWC2-AS1/miR-382-5p/FZD3 in granulosa cell (GCs) of polycystic ovary syndrome (polycystic ovarian syndrome, PCOS) patients and its molecular mechanism.Methods:Bioinformatics tools were used to predict the molecular mechanism of PCOS. The expressions of WWC2-AS1, miR-382-5p and FZD3 in serum and GCs of patients with PCOS and healthy controls were detected by qRT-PCR. The effects of WWC2-AS1/miR-382-5p/FZD3 on the proliferation and apoptosis of GCs were observed by CCK-8 and flow cytometry. The interaction between WWC2-AS1 and miR-382-5p, miR-382-5p and FZD3 was verified by double luciferase report experiment.Results:Compared with the control group, the expression of WWC2-AS1 and FZD3 in serum and GCs of PCOS patients was significantly up-regulated, while the expression of miR-382-5p was down-regulated. Silencing WWC2-AS1 could significantly promote the proliferation of GCs in PCOS and inhibit the apoptosis of GCs (all P<0.05) . There is a WWC2-AS1/miR-382-5p/FZD3 interaction network in PCOS, and miR-382-5p inhibitor or overexpressed FZD3 can partially reverse the regulatory effect of silent WWC2-AS1 on GCs in PCOS. Conclusion:This study shows that WWC2-AS1 regulates miR-382-5p and up-regulates FZD3, which promotes the proliferation of GCs and inhibits apoptosis in the progression of PCOS. WWC2-AS1/miR-382-5p/FZD3 may be an effective molecular target for the treatment of PCOS.
