Etiological diagnostic value of metagenomics next-generation sequencing in liver abscess
10.3760/cma.j.issn.1671-0282.2021.10.013
- VernacularTitle:宏基因二代测序技术对肝脓肿病原学诊断的价值
- Author:
Si SUN
1
;
Feixiang XU
;
Yi HAN
;
Mingming XUE
;
Zhenju SONG
;
Chaoyang TONG
Author Information
1. 复旦大学附属中山医院急诊科,上海 200032
- Keywords:
Liver abscess;
Metagenomic next-generation sequencing;
Etiological;
Reads;
Septic shock;
Blood culture;
Drainage solution culture;
Klebsiella pneumoniae
- From:
Chinese Journal of Emergency Medicine
2021;30(10):1229-1234
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the value of metagenomic next-generation sequencing (mNGS) in the pathogen diagnosis of liver abscess.Methods:A perspective study was performed in 35 hospitalized patients with liver abscess in Department of Emergency Medicine, Zhongshan Hospital, Fudan University from February 2020 to April 2021. Blood samples and abscess drainage fluid samples were detected by routine microbial culture and mNGS. Patients were divided into two groups according to whether they had septic shock or not. SPSS 25.0 was used for statistical analysis.Results:The overall positive rate of mNGS in blood samples and drainage fluid samples was significantly higher than that of routine microbial culture methods (blood: 67.6% vs. 15.2%, P<0.05; Drainage fluid: 100% vs. 55.2%, P<0.05). In 35 patients with liver abscess, 71.4% of the pathogens were Klebsiella pneumoniae. The sequence number of pathogenic pathogens detected by mNGS in abscess drainage fluid samples of patients in the shock group was significantly higher than that in the non-shock group ( P<0.05). Conclusions:The mNGS can quickly and accurately detect the pathogen of liver abscess, which can provide important etiological diagnostic for clinical treatment.
