Bone marrow mesenchymal stem cells protect myocardial ischemia-reperfusion injury by inducing regulatory T cells in mice
10.3760/cma.j.issn.1671-0282.2021.08.011
- VernacularTitle:骨髓间充质干细胞诱导调节性T细胞缓解心肌缺血-再灌注损伤
- Author:
Lingxiao PANG
1
;
Qian LI
;
Wei ZHU
;
Bin SHENG
;
Yongsheng YUAN
;
Yingwei OU
;
Rongcheng AN
Author Information
1. 浙江省人民医院 杭州医学院附属人民医院急诊医学科，杭州 310014
- Keywords:
Bone Marrow derived Mesenchymal Stem Cell;
Regulatory T cell;
Ischemia-reperfusion injury;
Inflammatory factors
- From:
Chinese Journal of Emergency Medicine
2021;30(8):973-978
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the protective effect and mechanism of bone marrow-derived mesenchymal stem cell (BM-MSC) on myocardial ischemia-reperfusion injury (MIRI) in mice.Methods:Twenty four C57 MIRI mice were randomly(random number) divided into four groups: SO group, RI group, MSC+RI group, and MSC + RI+ PC61 group. The ratio of Treg were detected by flow cytometry. Serum levels of CK, TNI, BNP, IL-10 and TGF-β were measured by ELISA. The histological changes of myocardium were observed by HE staining. The number of cardiomyocyte apoptosis was measured by TUNEL staining, and the area ratio of myocardial infarction were determined by TTC staining. One-way ANOVA was used to analyze the data.Results:In the MSC + RI group, the ratio of Treg and the levels of IL-10 and TGF-β were the highest, while CK, TNI and BNP were the lowest ( P<0.01) .The number of myocardial apoptotic cells, infarct size and tissue fibrosis were the least ( P<0.01) . Conclusions:MSC can induce the production of Treg, increase the release of anti-inflammatory cytokines IL-10 and TGF-β, and reduce the inflammatory injury after myocardial ischemia-reperfusion.
