Determination of formononetin and harmful elements of heavy metals in Jinji Pill
10.3760/cma.j.cn115398-20200524-00223
- VernacularTitle:金鸡丸中芒柄花素及重金属有害元素含量测定
- Author:
Tao CHENG
1
;
Yingchao WANG
;
Ke SHU
;
Tielin WANG
;
Ling CHANG
Author Information
1. 贵州省食品药品检验所中药室，贵阳 550004
- Keywords:
Formononetin;
Chromatography, liquid;
Spectrophotometry, atomic;
Jinji Pill;
Heavy metals;
Assay (TCD);
Quality control
- From:
International Journal of Traditional Chinese Medicine
2021;43(10):1006-1010
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To use the high performance liquid chromatography method to determine the content of formononetin in Jinji Pills and by using atomic absorption spectrophotometry,method to determine the harmful elements of heavy metal in Jinji Pills in orer to provide the scientific foundation for improving its quality standards and safety evaluation. Methods:Use Waters XBridge? C18 column (4.6 mm × 250 mm, 5 μm), set mobile phase at acetonitrile-1% phosphoric acid solution (27:73), flow rate 1.0 ml/min, column temperature 30 ℃, detection wavelength 249 nm, column temperature 30 ℃; Lead (Pb) and cadmium (Cd) was detected by graphite furnace method; arsenic (As) was detected by cold steam series graphite furnace method; copper (Cu) was detected by flame method; mercury (Hg) was detected by cold steam method.Results:The formononetin had a good linear relationship between 0.02-2.01 μg, the recovery rate was 98.5%, RSD was 1.53%. Lead (Pb) recovery rate was 103.6%, cadmium (Cd) recovery rate was 95.7%, arsenic (As) recovery rate was 92.4%, mercury (Hg) recovery rate was 104.9%, copper (Cu) recovery rate was 112.5%. Conclusion:This method is of accuracy, specificity, high sensitivity and good reproducibility, which could provide strong evidence for quality improvement and safety use of Jinji pill.
