Simultaneous determination of five ingredients in Taohe-Chengqi Decoction by UPLC-MS/MS
10.3760/cma.j.cn115398-20200715-00175
- VernacularTitle:超高效液相串联质谱法同时测定桃核承气汤中5种成分含量
- Author:
Jiayu GUO
1
;
Mengxi QIAN
Author Information
1. 南京中医药大学附属张家港医院药学部 215600
- Keywords:
Tao He Cheng Qi Tang;
Tandem mass spectrometry;
Amygdalin;
Cinnamic acid;
Rhein;
Emodin;
Glycyrrhizic acid;
Quality control;
Assay (TCD)
- From:
International Journal of Traditional Chinese Medicine
2021;43(9):893-897
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop the UPLC-MS/MS method for the determination of amygdalin, cinnamic acid, rhein, emodin and glycyrrhizic acid in Taohe-Chengqi Decoction simutaneously. Methods:The separation was performed on Supelco Discovery C18, and isocratic elution was carried out with mobile phase consisting of acetonitrile - 4 mmol/L ammonium formate. The mass spectrometer was operated in the positive and negative ionization electrospray (ESI) mode using multiple monitoring (MRM) to analize of five ingredients. The precursor to product ion transitions monitored for amygdalin, cinnamic acid, rhein, emodin and glycyrrhizic acid were m/z 458.2→296.0, 146.8→103.1, 283.7→239.9, 269.7→226.1 and 821.4→350.9, respectively. Results:Amygdalin, cinnamic acid, rhein, emodin and glycyrrhizic acid were analyzed, the linear ranges were 0.001 6-0.102 4, 0.001 6-0.102 4, 0.001 6-0.102 4, 0.000 8-0.051 2 and 0.000 4-0.256 0 ng, respectively. The r were 0.998 7, 0.999 1, 0.999 5, 0.998 9 and 0.998 6, respectively. The recovery of five analytes ranges from 97.33% to 105.33% and the Relative Standard Deviations were all below 2.69%. Conclusion:This UPLC-MS/MS method is exclusive, rapid and sensitivewhich could be applied for the determination of amygdalin, cinnamic acid, rhein, emodin and glycyrrhizic acid in Taohe-Chengqi Decoction.
