Optimization of supercritical CO 2 extraction of red ginseng polysaccharide by response surface methodology and its antioxidant activity in vitro
10.3760/cma.j.cn115398-20210113-00126
- VernacularTitle:响应面法优化超临界CO 2萃取红参多糖工艺及体外抗氧化活性研究
- Author:
Jing SUN
1
;
Xinxin MENG
;
Ya JING
;
Yijun SONG
;
Qiao YANG
Author Information
1. 陕西中医药大学药学院，咸阳 712046
- Keywords:
Total polyphenols from red ginseng;
Supercritical CO 2;
Extraction;
Response surface method
- From:
International Journal of Traditional Chinese Medicine
2021;43(9):887-892
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Response surface methodology was used to optimize the optimal extraction conditions of red ginseng polysaccharide and to study the antioxidant activity of red ginseng polysaccharide.Methods:Supercritical CO 2 extraction method was used to extract polysaccharides from red ginseng. The effects of solid-liquid ratio, extraction time, extraction temperature and extraction pressure on the extraction of polysaccharides from red ginseng were investigated. Box-behnken Design method was used to optimize the extraction process of red ginseng polysaccharide, and Logit method was used to calculate the semi-inhibitory concentration of red ginseng polysaccharide on DPPH clearance (half maximal inhibitory concentration, IC 50). Results:The optimal extraction conditions were as follows: extraction temperature 61.12 ℃, extraction pressure 20.64 MPa, extraction time 128.37 min, solid-liquid ratio 1∶25.61 g/ml, and the extraction yield of red ginseng polysaccharide was 36.89%. The results of three groups of repeatability tests showed that the relative error of polysaccharide yield of red ginseng was in the range of 5%. When the mass concentration of red ginseng polysaccharide was 25 μg/ml, it had better antioxidant activity and IC 50 was 10.97 μg/ml. Conclusion:The optimized extraction conditions of red ginseng polysaccharide were reasonable and reliable, and the antioxidant activity of red ginseng polysaccharide was strong, which could provide reference for the follow-up research.
