Study on intestinal flora characteristics of asthmatic children based on theory of lung and large intestine being interior-exterior
10.3760/cma.j.cn115398-20200728-00310
- VernacularTitle:基于"肺与大肠相表里"的哮喘儿童肠道菌群特征研究
- Author:
Jia CHEN
1
;
Yingwu CHE
;
Jie LIN
;
Yiqin WANG
;
Kexing SUN
;
Fang LIU
Author Information
1. 上海交通大学医学院附属上海儿童医学中心中医科 200127
- Keywords:
Asthma;
Intestinal flora;
Child;
Lung and large intestine being interior-exterior
- From:
International Journal of Traditional Chinese Medicine
2021;43(8):744-750
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the characteristics of intestinal flora in children with asthma from the theory of "lung and large intestine being interior-exterior" , and to provide an experimental basis for the study of "lung and intestine axis" .Methods:Based on 16S rDNA high-throughput sequencing technology, stool samples from children with 30 asthma and 28 healthy children were enrolloed and sequenced. All patients are from january 2018 to April 2018, Shanghai Children's Medical Center affiliated to Shanghai Jiaotong University Medical College and Oriental Hospital Affiliated to Tongji University. Statistical methods such as Principal component analysis (PCA), LEfSe analysis, linear discriminant analysis (LDA), and non-metric multidimensional scale analysis (NMDS) were used to analyze intestinal flora characteristics of asthma children.Results:The number of Intestinal flora operating taxon unit (OTU) in asthmatic group (141.96 ± 27.42 vs. 164.54 ± 50.58, P=0.048), Shannon index (2.49 ± 0.51 vs. 2.80 ± 0.56, P=0.044) were significantly lower than those of healthy children group, and Simpson index (0.18 ± 0.06 vs. 0.13 ± 0.07, P=0.010) was significantly higher than that of healthy children. There was no significant difference in intestinal flora abundance between asthmatic children and healthy children ( P>0.05), but there was a decreasing trend. Further differential intestinal flora analysis showed that there were differences in bacterial abundance between children with asthma and healthy children. Conclusion:Children with asthma showed high related with intestinal flora disorders, referring that asthma maybe treated when intestinal flora is treated, which provides a reference for the study of the relationship between lung and intestine from the perspective of intestinal flora.
