Mechanism of UGT2A3 differentially expression in the occurrence and early diagnosis of colorectal cancer
10.3760/cma.j.cn121382-20210103-00302
- VernacularTitle:UGT2A3差异表达在结直肠癌发生和早期诊断中的作用机制
- Author:
Hongfeng LI
1
;
Junfang ZHAO
;
Xuewen CHEN
;
Lin SONG
;
Nan BU
Author Information
1. 天津市中医药研究院附属医院检验科 300190
- Keywords:
UGT2A3;
miRNA;
Colorectal cancer;
Early diagnosis;
Regulatory mechanism
- From:
International Journal of Biomedical Engineering
2021;44(3):184-191
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role and regulatory mechanism of UGT2A3 differential expression in colorectal carcinogenesis.Methods:Nine CRC datasets were downloaded from GEO database and TCGA. R language was used to analyze the differential expression of UGT2A3 in cancer and normal tissues. According to the expression level of UGT2A3 in TCGA, the top 20 samples with the highest expression and the lowest expression were selected from normal tissues and CRC tissues, respectively. The abundance of immune cells and immune enrichment score were compared, the differentially expressed genes and differentially expressed miRNAs were screened, and the pathway enrichment analysis of differentially expressed genes was performed.Results:UGT2A3 was down regulated in all 9 CRC datasets. In all sample types, compared with the UGT2A3 high expression group, the UGT2A3 low expression group had significantly higher ImmuneScore, EstimateScoreandStromalScore, and had higher abundance of immune cells (except memory B cells). In normal tissues, the differential expression of UGT2A3 mainly affects cancer-related pathways, while in tumor tissues, it mainly affects metabolic pathways. miR-194-2, miR-224 and miR-551b were differentially expressed in all groups, which were considered as potential UGT2A3 upstream regulatory genes in CRC.Conclusions:UGT2A3, miR-194-2, miR-224 and miR-551b can be used as potential biomarkers for the diagnosis of CRC.
