Determination of zolpidem tartrate tablets after radiation by UHPLC
10.12206/j.issn.1006-0111.202110019
- VernacularTitle:UHPLC法测定辐射后的酒石酸唑吡坦片含量
- Author:
Wen ZHANG
1
,
2
;
Xinhui HUANG
2
;
Xingrui YANG
2
;
Tingting ZHOU
1
,
2
;
Jianyi GAO
3
;
Yongzhi LI
3
Author Information
1. School of Pharmacy, Anhui University of Traditional Chinese Medicine, Hefei 230012, China
2. School of Pharmacy, Naval Military Medical University, Shanghai 200433, China.
3. Chinese Astronaut Research and Training Center, Beijing 100094, China.
- Keywords:
zolpidem tartrate;
ultra high performance liquid chromatography;
content determination;
radiation
- From:
Journal of Pharmaceutical Practice
2022;40(1):62-65
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a UHPLC method for the determination of zolpidem tartrate tablets after radiation, and to investigate the effect of different radiation doses on the content of zolpidem tartrate tablets. Methods Ultra high performance liquid chromatography was used. The content of zolpidem tartrate tablets irradiated by γ-ray was determined. Using C18 column, acetonitrile methanol-0.05 mol/L phosphoric acid solution (the pH value as 5.5 with triethylamine) (18∶26∶56) was used as the mobile phase. The flow rate was 0.7 ml/min, and the detection wavelength was 254 nm. Results The method validation showed good linearity in the concentration range of 5-80 μg/ml (r=0.999 6); The average recovery was 98.2%, RSD was 1.72%, and the repeatability was 0.87%. The contents of zolpidem tartrate were 105.1%, 106.4%, 102.7% and 105.4% under 0, 8, 25 and 80 kGy radiation. Conclusion UHPLC has accurate results with short analysis cycle in this study. It is suitable for the determination of zolpidem tartrate tablets after radiation. The content of zolpidem tartrate tablets remained basically unchanged after radiation.
