Rapid prediction of antioxidant activity in aqueous extract solutions of melastoma dodecandrum by ultraviolet spectroscopy
10.12206/j.issn.1006-0111.202101016
- VernacularTitle:地稔水提液抗氧化活性的紫外光谱快速预测方法
- Author:
Cheng JIANG
1
;
Pingcui XU
1
;
Renjie WU
1
;
Xiaoqing ZHANG
2
;
Liping CHEN
2
;
Nani WANG
1
Author Information
1. Tongde Hospital of Zhejiang Province, Hangzhou 310012, China.
2. Lishui Traditional Chinese Medicine Hospital, Lishui 323000, China.
- Keywords:
Melastoma dodecandrum;
aqueous extract solution;
antioxidant activity;
ultraviolet spectroscopy;
partial least squares regression
- From:
Journal of Pharmaceutical Practice
2022;40(1):48-52
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a rapid prediction method of the antioxidant activity in aqueous extract solutions of Melastoma dodecandrum based on ultraviolet spectroscopy and partial least squares regression algorithm. Methods The DPPH free radical scavenging effect was used to characterize the antioxidant activity of aqueous extract solutions of Melastoma dodecandrum. The ultraviolet spectra of 190-600 nm were collected. The partial least squares regression model of antioxidant activity was established after optimizing the wavelength range and preprocessing method. The software was devised using Visual Basic as the integrated development environment to provide a convenient tool for the rapid determination of antioxidant activity. Results The optimal partial least squares regression model was established based on 200-290 nm as wavelength range and unit variance scaling as preprocessing method. The correlation coefficient of calibration, root mean square error of estimation, root mean square error of cross-validation was 0.887, 2.20% and 2.17%, respectively. The correlation coefficient of validation, root mean square error of prediction was 0.868, 2.08%. The average predicted recovery was 100.1±2.3%. With the predictive function in the software, the antioxidant activity of aqueous extract solution of Melastoma dodecandrum can be calculated automatically within 2 s after collecting the ultraviolet spectra. Conclusions This study provides a rapid method for the prediction of antioxidant activity in aqueous extract solutions of Melastoma dodecandrum.
