Effect of exposure to low concentrations of benzene on miR-223 and miR-155 expression in peripheral blood lymphocytes
10.19485/j.cnki.issn2096-5087.2022.01.003
- Author:
WANG Aihong
;
LI Xiaohai
;
LENG Pengbo
;
DUAN Donghui
;
FANG Lanyun
;
ZHANG Dandan
- Publication Type:Journal Article
- Keywords:
benzene exposure；microRNA；peripheral blood lymphocyte；oxidative damage
- From:
Journal of Preventive Medicine
2022;34(1):11-16
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the effect of exposure to low concentrations of benzene on miR-155 and miR-223 expression in peripheral blood lymphocytes among workers with benzene exposure.
Methods :A hundred male employees at a risk of exposure to benzene (the exposed group) were randomly sampled from two small metal products manufacturing enterprises and one medium-sized chemical raw material and chemical products manufacturing enterprise in Ningbo City, Zhejiang Province, and 60 age-matched male employees without benzene exposure were randomly selected as the unexposed group. Age, body mass index ( BMI ), smoking status, alcohol consumption, disease history, medication history and routine blood testing results of subjects were collected using a questionnaire survey. The 8-hour time weighted average concentration ( CTWA ) of benzene was measured in the workplace using thermal desorption gas chromatography, and the urine 8-hydroxy-2' deoxyguanosine ( 8-OHdG ) levels were determined using high-performance liquid-chromatography tandem mass spectrometry (HPLC-MS/MS). The miR-155 and miR-223 expression was quantified in peripheral blood lymphocytes using quantitative fluorescent reverse transcription-polymerase chain reaction assay, and the factors affecting miR-155 and miR-223 expression were identified using multivariable logistic regression analysis.
Results :The subjects in the exposed group had a mean age of ( 31.17±7.30 ) years, and were exposed to low concentrations of benzene ( CTWA, 0.05 to 0.30 mg/m3 ) , while the subjects in the unexposed group had a mean age of ( 32.52±6.15 ) years. There were no significant differences between the exposed and unexposed groups in terms of age, BMI, proportion of smokers or proportion of alcohol consumers ( P>0.05 ). There was no significant difference in the median relative miR-155 expression between the exposed and unexposed groups ( 0.953 vs. 1.293, P>0.05 ), and lower median relative miR-223 expression was quantified in the exposed group than in the unexposed group ( 0.540 vs. 1.433, P<0.05 ). Multivariable logistic regression analysis revealed that down-regulation of miR-223 expression correlated with exposure to benzene ( OR=2.719, 95%CI: 1.308-5.651 ).
Conclusion :Down-regulation of miR-223 expression may be associated with exposure to low concentrations of benzene.
- Full text:低浓度苯暴露与外周血淋巴细胞miR-223和miR-155表达的关联研究.pdf
