Differences in the chemical composition of Dendrobium officinale Kimura et Migo and Dendrobium crepidatum Lindl based on UPLC-Q-TOF-MS/MS and metabolomics
10.16438/j.0513-4870.2021-0939
- VernacularTitle:基于UPLC-Q-TOF-MS/MS代谢组学的铁皮石斛和玫瑰石斛化学成分差异研究
- Author:
Gang-gui LOU
1
;
Jie XIA
1
;
Jian YANG
2
;
Hong-peng WANG
3
;
Zong-suo LIANG
1
;
Yi XIAO
4
;
Zhen-da LI
4
;
Yu ZHANG
5
;
Zhi-chao LIU
6
;
Wan-li SHI
3
;
Xiao-dan ZHANG
1
;
Dong-feng YANG
1
Author Information
1. College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, Hangzhou 310018, China
2. State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
3. School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China
4. Ningbo Yizhonghe Medical Plant Research Institute Co., Ltd., Ningbo 315010, China
5. Yunnan Yelantang Biological Technology Co., Ltd., Kunming 650114, China
6. Laboratory of Traditional Chinese Medicine, Lu'an Academy of Agricultural Sciences, Lu'an 237000, China
- Publication Type:Research Article
- Keywords:
italic>Dendrobium officinale Kimura et Migo;
italic>Dendrobium crepidatum Lindl;
ifferent parts;
UPLC-Q-TOF-MS/MS;
ifferential metabolites
- From:
Acta Pharmaceutica Sinica
2021;56(12):3331-3344
- CountryChina
- Language:Chinese
-
Abstract:
italic>Dendrobium officinale Kimura et Migo is a rare Chinese herbal medicine, while Dendrobium crepidatum Lindl is a local medicine in Yunnan, both of which have the function of nourishing yin and stomach. To reveal the differences in chemical composition between the two species, ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry (UPLC-Q-TOF-MS/MS) was used to analyze the chemical composition of stems and leaves of D. officinale and D. crepidatum. Principal component analysis (PCA) and partial least squares discriminant analysis (OPLS-DA) were used to determine the differences in metabolites between species and parts of Dendrobium. Fifty-eight chemical compounds were identified in the two species. Analysis indicated that the side ring of alkaloids connected with nitrogen was readily cleaved during analysis. The results of PCA analysis showed that the stems and leaves of D. officinale and D. crepidatum could be easily differentiated, and the chemical constituents of D. officinale and D. crepidatum were significantly different. OPLS-DA analysis showed that there were 16 metabolite differences between the stems and 22 differences in metabolites between the leaves of D. officinale and D. crepidatum. The main metabolite differences in components between the two Dendrobium species were dendrocrepidine B, dendrocrepidine C and dendrocrepine. There were 14 differences in metabolites between the stems and leaves of D. crepidatum. In conclusion, the chemical compositions of D. officinale and D. crepidatum are quite different; the small molecular compounds of D. officinale are mainly terpenoids and flavonoids, and the content of alkaloids is low. There is no significant difference between stem and leaf. In contrast, D. crepidatum is mainly composed of alkaloids and terpenoids, with crepidamine and dendrocrepine as its unique components, and there are great differences in the components between stems and leaves. This study provides a theoretical basis for the development and utilization of Dendrobium resources.
