Different development phase of transcriptome analysis from Crataegus pinnatifida Bge. and cloning, structure and function prediction of squalene epoxidase involved in triterpenic acid biosynthesis
10.16438/j.0513-4870.2021-0665
- VernacularTitle:山楂果实转录组分析及三萜合成关键酶基因SQE的克隆与生信分析
- Author:
Jun-xian WU
1
;
Rui XU
1
;
Min-zhen YIN
1
;
Jing LI
1
;
Han-wen YU
1
;
Meng-li LIU
1
;
Hua-sheng PENG
2
,
3
;
Liang-ping ZHA
1
,
4
Author Information
1. School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China
2. State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
3. Research Unit of DAO-DI Herbs, Chinese Academy of Medical Sciences, 2019RU57, Beijing 100700, China
4. Institute of Conservation and Development of Traditional Chinese Medicine Resources, Anhui Academy of Chinese Medicine, Hefei 230012, China
- Publication Type:Research Article
- Keywords:
italic>Crataegus pinnatifida;
ifferent periods;
active ingredient;
gene expression
- From:
Acta Pharmaceutica Sinica
2021;56(12):3313-3324
- CountryChina
- Language:Chinese
-
Abstract:
italic>Crataegus pinnatifida is a traditional Chinese medicine, which contains organic acids, triterpenoid acids and other active components, has important medicinal and edible value. In order to study the difference of gene expression level in different developmental stages of hawthorn and explore the genes of active ingredient biosynthesis in Crataegus pinnatifida, high-throughput Illumina HiSeq 2000 technology were used to conduct transcriptome sequencing and bioinformatics analysis on Crataegus pinnatifida fruits from the same origin at different developmental stages. 78 496 Unigenes with an average length of 941 nt were obtained by Trinity software. Among them, 58 395 Unigenes can be annotated by NR, NT, Swiss prot, KEGG, COG, GO and other public databases. KEGG pathway analysis showed that 52 Unigenes encoding 15 key enzymes involved in the citric acid cycle. There are 62 Unigenes were involved in the triterpene biosynthesis pathway of Crataegus pinnatifida. Two key enzymes SQE of triterpenoid metabolism pathway in Crataegus pinnatifida were cloned and performed bioinformatic analysis. The results showed that ORF of CpSQE1 and CpSQE2 were 1 594 bp and 1 597 bp, respectively, encoding 530 and 531 amino acids. The molecular weight of proteins was 57.6 kDa and 57.5 kDa. Bioinformatics analysis showed that both CpSQE1 and CpSQE2 proteins have a PLN02985 superfamily conserved domain, belonging to the squalene monooxygenase superfamily. The phylogenetic tree shows that CpSQE1 and CpSQE2 are clustered together with SQE with squalene epoxidase function in other plants. This study provides a research basis for further exploring the key genes in the biosynthesis process of hawthorn active ingredients and analyzing the regulation pathway of its active ingredient biosynthesis.
