Determination of ibotenic acid and muscimol in wild mushroom by dansyl chloride derivatization-liquid chromatography-tandem mass spectrometry
10.19485/j.cnki.issn2096-5087.2021.12.007
- Author:
XU Xiaomin
;
CAI Zengxuan
;
XU Jiaojiao
;
ZHENG Yibin
;
CHEN Qing
;
HAN Jianlong
- Publication Type:Journal Article
- Keywords:
wild mushroom；ibotenic acid；muscimol；dansyl chloride；liquid chromatography-tandem mass spectrometry
- From:
Journal of Preventive Medicine
2021;33(12):1223-1227
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To develop an analytical method of ibotenic acid (IBA) and muscimol (MUS) in wild mushroom by dansyl chloride (DNSCl) derivatization-liquid chromatography-tandem mass spectrometry (LC-MS/MS), and to provide technical support for etiological identification of mushroom poisoning events.
Methods :The sample was extracted with hydrochloric acid solution, derived by bimolecular DNSCl, diluted and inorganic salts precipitated with acetonitrile. The extract was separated by a waters XBridgeTM BEH C18 column and measured by LC-MS/MS.
Results :The limits of detection for IBA and MUS in wild mushroom were 0.15 mg/kg and 0.1 mg/kg, respectively. Good linear relationship was obtained for IBA and MUS at the range of 0.5-250 mg/kg with the correlation coefficient of 0.997 and 0.999, respectively. The average recoveries at three spiking levels were 84.5%-102.0% with relative standard deviations (RSDs, n=6) of 4.7%-8.6% for IBA. The average recoveries were 88.6%-95.4% with RSDs (n=6) of 4.9%-7.5% for MUS.
Conclusion :The optimized sample extraction and bimolecular DNSCl derivatization conditions can achieve rapid and accurate analysis of IBA and MUS in wild mushroom poisoning sample.
- Full text:丹磺酰氯衍生-液相色谱-串联质谱法检测野生菌鹅膏蕈氨酸和毒蝇母.pdf
