Preliminary Study on Effects of Solamargine on the Growth and Apoptosis of Human Hepatocarcinoma Cells HepG 2 and Its Mechanism
- VernacularTitle:澳洲茄边碱对人肝癌HepG2细胞生长和凋亡的影响及机制初探
- Author:
Jingjing WU
1
;
Wenjuan ZHANG
2
;
Junhong ZHANG
1
;
Yating ZHANG
2
;
Li LI
1
;
Yue LU
1
;
Jian’an WEI
1
;
Ling HAN
3
Author Information
1. Research Team of Molecular Biology and System Biology of Chinese Medicine，the Second Affiliated Hospital of Guangzhou University of Chinese Medicine，Guangzhou 510120，China
2. The Second Clinical College of Guangzhou University of Chinese Medicine，Guangzhou 510120，China
3. State Key Laboratory of Dampness Syndrome of Chinese Medicine，the Second Affiliated Hospital of Guangzhou University of Chinese Medicine，Guangzhou 510120，China
- Publication Type:Journal Article
- Keywords:
Solamargine;
Human hepatocarcinoma cells HepG 2;
Growth;
Apoptosis;
AMPK signaling pathway
- From:
China Pharmacy
2021;32(24):2963-2969
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To ex plore the effects of solamargine on the growth and apoptosis of human hepatocarcinoma cells HepG2 and its underlying mechanism. METHODS ：The effects of 0（blank group ）-12 μmol/L solamargine treatment of 24，48 h on survival rate of HepG 2 cells were investigated. The effects of 0（blank group ），6 μmol/L solamargine treatment of 10 days on cell clone formation were also investigated. The effects of 0（blank group ），4，6，8 μmol/L solamargine for 24 h on the apoptotic rate of cells，mRNA expression of Bcl- 2，Bax and caspase- 3， protein expression of Bcl- 2 and cleaved caspase- 3 as well as ratio of p-AMPKα to AMPKα were all tested. The effects of AMPK inhibitor as compound C on the protein expression of AMPKα and Bcl- 2 in cells were investigated after treated with 6 μmol/L solamargine for 24 h. RESULTS ：Compared with 020-39318678。E-mail：wujingjing6028@gzucm.edu.cn blank group ，1-12 μ mol/L solamargine for 24，48 h could significantly decrease the survival rates of cells （P＜0.05）in a concentration-dependent manner ；IC50 of them were 8.310 and 7.996 μmol/L，respectively；the rate of cell clone formation was decreased significantly after treated with 6 μmol/L solamargine for 10 days（P＜0.05）. The apoptotic rate of HepG 2 cells，mRNA expression of Bax and caspase- 3，protein expression of cleaved caspase-3（except for 8 μmol/L）as well as ratio of p-AMPKα to AMPKα（except for 8 μmol/L）were all increased significantly after treated with 6，8 μmol/L solamargine（P＜0.05）；mRNA and protein expression of Bcl- 2 were decreased significantly （P＜ 0.05）；the changes of some indexes were in a concentration-dependent manner. The compound C could inhibit protein expression of AMPKα，and reverse the inhibitory effect of solamargine on Bcl- 2 protein. CONCLUSIONS ：Solamargine can inhibit the proliferation of HepG 2 cells and induce apoptosis ，the mechanism of which may be associated with activating AMPK signaling pathway.
