28-hydroxy-3-oxoolan-12-en-2-oic acid and miR-451 synergistically inhibit the proliferation and migration of gastric cancer AGS cells and its possible mechanism

Sun Hui; Hua Weiwei; Chen Xiwen; LI Yajuan; Qin Wei; Yin Zixin; Zhao Ya; Liu Yanqing; Qian Yayun

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28-hydroxy-3-oxoolan-12-en-2-oic acid and miR-451 synergistically inhibit the proliferation and migration of gastric cancer AGS cells and its possible mechanism

VernacularTitle:齐墩果烷型五环三萜协同miR-451抑制胃癌AGS细胞的增殖和迁移及其可能的机制
Author: SUN Hui ¹ ; HUA Weiwei ¹ ; CHEN Xiwen ¹ ; LI Yajuan ¹ ; QIN Wei ¹ ; YIN Zixin ¹ ; ZHAO Ya ¹ ; LIU Yanqing ¹ ; QIAN Yayun ¹
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1. Department of Integrated Traditional Chinese and Western Medicine, Medical College of Yangzhou University, Yangzhou 225009, Jiangsu, China
Publication Type:Journal Article
Keywords: 齐墩果烷型五环三萜；miR-451；胃癌；AGS细胞；增殖；迁移；凋亡；mTOR
From: Chinese Journal of Cancer Biotherapy 2021;28(11):1081-1086
CountryChina
Language:Chinese
Abstract: [摘要] 目的：探索南蛇藤提取物齐墩果烷型五环三萜（28-hydroxy-3-oxoolean-12-en-2-oic acid）协同miR-451对人胃癌AGS细胞增殖、迁移的影响及其可能的分子机制。方法：用miR-451过表达慢病毒感染AGS细胞，并用盐酸多西环素（DOX）10或100 ng/ml诱导24 h，构建过表达miR-451的细胞AGS/miR-451+。采用10、20、40、80、160 μmol/L的齐墩果烷型五环三萜处理AGS/miR-451+细胞，MTT法、划痕实验分别检测细胞增殖和迁移能力的变化，WB法检测细胞中mTOR通路及凋亡相关蛋白表达水平的变化。结果：成功构建过表达miR-451的AGS/miR-451+细胞。与未加药对照组相比，齐墩果烷型五环三萜处理后AGS/miR-451+细胞的增殖抑制率均呈时间和浓度依赖性升高（P<0.05或P<0.01），细胞迁移率均显著降低（P<0.05或P<0.01）。齐墩果烷型五环三萜处理组细胞中，mTOR 信号通路相关蛋白的表达量均有所降低（P<0.05或P<0.01）；凋亡相关蛋白中，Bcl2的表达量下降，BAX、caspase-3、caspase-1及细胞色素c的表达量升高（P<0.05或P<0.01）。结论：齐墩果烷型五环三萜能够协同miR-451抑制人胃癌AGS细胞的增殖与迁移，其机制可能与影响凋亡和mTOR信号通路相关蛋白的表达有关。
Full text:20211105.pdf