Study on Reversal Effect of Quercetin on Human Cervical Squamous Carcinoma Cisplatin-resistant Cell Line SiHa/DDP
- VernacularTitle:槲皮素对人宫颈鳞癌顺铂耐药细胞SiHa/DDP的逆转作用研究
- Author:
Lei FENG
1
;
Jianmin YANG
1
;
Ran YANG
1
;
Li SUN
1
;
Lili DONG
1
;
Qingwei ZHANG
2
Author Information
1. Dept. of Gynaecology，Nanyang Municipal Central Hospital，Henan Nanyang 473000，China
2. Dept. of Gynaecology，the First Affiliated Hospital of Luohe Medical College，Henan Luohe 462000，China
- Publication Type:Journal Article
- Keywords:
Quercetin;
Cervical squamous carcinoma;
SiHa/DDP cells;
PI3K/Akt/mTOR signaling pathway;
Drug resistance
- From:
China Pharmacy
2021;32(23):2875-2879
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To study the reversal effect of quercetin on human cervical squamous carcinoma cisplatin-resistant cell line SiHa/DDP. METHODS ：The drug resistance index of cisplatin to SiHa/DDP cells ，and the reversal resistance multiple of quercetin to SiHa/DDP cells were determined. The effects of quercetin （0.005 μg/mL），cisplatin（2.5 μg/mL），cisplatin combined with quercetin （2.5 μg/mL cisplatin+0.005 μg/mL quercetin），quercetin combined with pathway inhibitor（0.005 μg/mL quercetin+ 20 nmol/L rapamycin ）on the apoptotic rate of SiHa/DDP cells were investigated ，as well as its effects on the expression of phosphatidylinositol 3-kinase （PI3K）/protein kinase B （Akt）/mammalian rapamycin target protein （mTOR） signaling pathway related proteins （PI3K，Akt，mTOR，P-gp，p70S6K）. RESULTS ：The resistance index of cisplatin to SiHa/DDP cells was 5.19， and reversal resistance multiple of quercetin to SiHa/DDP cells was 4.00. Compared with cisplatin alone and quercetin alone ， cisplatin combined with quercetin ，quercetin combined with rapamycin could significantly increase the apoptotic rate of SiHa/DDP cells（P＜0.05），while decreased the phosphorylation of Akt ，mTOR and p 70S6K protein as well as the expression of P-gp protein （P＜0.05）. CONCLUSIONS ：Quercetin can effectively reverse drug resistance of SiHa/DDP cells to cisplatin ，which may be associated with inhibiting the expression of the protein related to PI 3K/Akt/mTOR signaling pathway.
