A retrospective analysis of 2 728 HIV antibody screening positive samples
10.3969/j.issn.1006-2483.2021.06.017
- VernacularTitle:2 728例HIV抗体筛查阳性样本检测结果的回顾性分析
- Author:
Yan XING
1
;
Cong LIU
2
;
Fanghua MEI
2
;
Meng GUO
2
;
Kun CAI
2
Author Information
1. Huanggang City Center for Disease Control and Prevention , Huanggang , Hubei 438000, China
2. Hubei Provincial Center for Disease Control and Preventio , Wuhan 430079, China
- Publication Type:Journal Article
- Keywords:
HIV;
S/CO ratio;
Western blot (WB);
Nucleic acid tests
- From:
Journal of Public Health and Preventive Medicine
2021;32(6):71-74
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the positive results of HIV antibody screening in the laboratory of AIDS confirmation center of Hubei Provincial Center for Disease Control and Prevention from 2014 to 2020, and to provide a basis for improving detection strategies. Methods A total of 2 728 primary screening positive specimens received by the laboratory of Hubei confirmation center from 2014 to 2020 were retested with two reagents. Specimens with at least one reactive result were confirmed with western blot (WB). The samples with uncertain or negative WB results were further confirmed by nucleic acid quantitative detection. The test results were analyzed retrospectively. Results A total of 2 297 specimens with positive retest results were confirmed by WB, with a positive rate of 93.47%. The highest proportion of patients was from medical institutions. The positive rate detected by 4 diagnostic kits was apparently higher in S/CO＞10 cases than that in S/CO≤10, and the difference was statistically significant (P < 0.05). Of the 19 samples with inconsistent results in ELISA, 1 was confirmed as positive by WB, while 12 were negative both by WB and nucleic acid detection. Among 68 samples with uncertain WB results, 49 cases had nucleic acid quantitative results > 5 000cps / ml, and 12 cases were TND. 13 of the 30 WB negative samples had nucleic acid test results＞5 000CPs/mL . Conclusions The coverage of HIV screening laboratories in hospitals at all levels should be further increased to find more HIV infected persons. The anti-HIV ELISA S/CO ratio is correlated with the positive results confirmed by western blot. Therefore, ELISA S/CO ratio can be used to predict anti-HIV antibody positivity. For samples with uncertain or negative WB detection, supplemental nucleic acid test should be carried out timely for early diagnosis.
