Molecular cloning and characterization of a novel aspartyl aminopeptidase from Trichinella spiralis
https://doi.org/10.47665/tb.38.3.085
- Author:
Sun, X.Y.
1
;
Ma, K.N.
1
;
Bai, Y.
1
;
Liu, R.D.
1
;
Long, S.R.
1
;
Zhang, X.
1
;
Jiang, P.
1
;
Cui, J.
1
;
Wang, Z.Q.
1
Author Information
1. Department of Parasitology, Medical College, Zhengzhou University, 40 Daxue Road, Zhengzhou 450052, Peoples Republic of China
- Publication Type:Journal Article
- Keywords:
Trichinella spiralis;
aspartyl aminopeptidase;
enzyme activity;
RNA interference
- From:Tropical Biomedicine
2021;38(No.3):420-434
- CountryMalaysia
- Language:English
-
Abstract:
Trichinellosis is an important zoonotic parasitic disease worldwide and is principally caused by ingesting animal meat containing Trichinella infective larvae. Aspartyl aminopeptidase is an intracytoplasmic metalloproteinase that specifically hydrolyzes the N-terminus of polypeptides free of acidic amino acids (aspartic acid and glutamate), and plays an important role in the metabolism, growth and development of organisms. In this study, a novel T. spiralis aspartyl aminopeptidase (TsAAP) was cloned and expressed, and its biological properties and roles in worm growth and development were investigated. The results revealed that TsAAP transcription and expression in diverse T. spiralis stages were detected by RT-PCR and Western blotting, and primarily localized at cuticle, stichosome and intrauterine embryos of this nematode by immunofluorescence test. rTsAAP has the enzymatic activity of native AAP to hydrolyze the substrate H-Glu-pNA. There was a specific binding between rTsAAP and murine erythrocyte, and the binding site was localized in erythrocyte membrane proteins. Silencing of TsAAP gene by specific dsRNA significantly reduced the TsAAP expression, enzymatic activity, intestinal worm burdens and female fecundity. The results demonstrated that TsAAP participates in the growth, development and fecundity of T. spiralis and it might be a potential target molecule for anti-Trichinella vaccines.
- Full text:8.2021my1285.pdf
