Effects of Sedimentary Type Limonitum on 6-keto-PGF1α，TXB2 and Other Related Indexes and Metal Ions in Blood of Warfarin Hemorrhage Model Rats
10.13422/j.cnki.syfjx.20210315
- VernacularTitle:沉积型禹余粮对华法林出血模型大鼠血液中6-keto-PGF1α，TXB2等相关指标及金属离子的影响
- Author:
Sheng-jin LIU
1
;
Chao-ying WU
1
;
Yu-lu MA
1
;
Fang FANG
1
;
Yong BIAN
1
;
Wen-guo YANG
1
;
Hui YAN
1
;
Zhi-jie ZHANG
2
;
Wu-li-ji AO
3
;
Jin-ao DUAN
1
Author Information
1. School of Pharmacy，Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization，National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine，State Administration of Traditional Chinese Medicine Key Laboratory of Chinese Medicinal Resources Recycling Utilization，Nanjing University of Chinese Medicine，Nanjing 210023，China
2. Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100700，China
3. Inner Mongolia University for Nationalities，Tongliao 028000，China
- Publication Type:Research Article
- Keywords:
mineral Chinese medicine（MCM）;
Limonitum;
hemostatic effects;
Warfarin;
metal elements;
material basis;
mechanism of action
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(6):105-112
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of sedimentary type Limonitum on hemostatic indexes in blood and metal ions in serum of rats with hemorrhage. Method:The hemorrhagic rat models were established by warfarin sodium. The experimental animals were divided into control group，model group，powder group and water decoction group. On day 15 from drug administration， the contents of 6-keto prostaglandin F1α（6-keto-PGF1α），thromboxane B2（TXB2），arachidonic acid（AA），endothelin 1（ET-1），platelet activating factor（PAF），P-selectin（PS），and Ca2+ in the whole blood of rats in each group were determined by enzyme-linked immunosorbent assay（ELISA）. The contents of Na，Mg，K，Ca，Fe，Al，Li，Be，Ti，V，Cr，Mn，Co，Ni，Cu，Zn，As，Sr，Cd，Sn，Sb，Ba，and Pb in serum samples were determined by inductively coupled plasma optical emission spectrometer（ICP-OES） and inductively coupled plasma mass spectrometry（ICP-MS）. Result:Compared with the model group，the content of 6-keto-PGF1α was reduced in the powder group and water decoction group （P<0.05），and the contents of TXB2，AA，ET-1，PAF，PS，Ca2+ were significantly increased（P<0.01），with a positive and beneficial regulatory effect. In the powder group， 10 kinds of metal elements in serum of rats were significantly and positively regulated： Na，K，Ca，Fe，Li，Ti，V，Co，Cu，and Zn（P<0.05，P<0.01）. In the water decoction group， 10 metal elements with significant positive regulation were as follows： Na，K，Ca，Fe，Li，V，Ni，Cu，Zn，and Sr（P<0.05，P<0.01）. In addition，the content of Cr（P<0.01） in the powder group and Cr（P<0.01），Pb（P<0.05） in the water decoction group were significantly reduced. Conclusion:The powder and water decoction of sedimentary type Limonitum had definite and positive intervention effect on warfarin hemorrhage model rats，which could play a coagulation role by enhancing the vasoconstriction ability，promoting the activation of platelets，and increasing the platelet aggregation rate and blood viscosity. The metal elements such as Na，K，Ca，Fe，Li，Ti，V，Co，Cu，Zn，Ni and Sr may be the material basis for sedimentary type Limonitum to exert hemostatic effect. According to the above indicators，the intervention effect of powder group and decoction group was basically the same.
