Serum Metabonomics Analysis of Colitis Associated Cancer Mice Intervened by Hedyotis diffusa Based on UPLC-Q-TOF-MS
10.13422/j.cnki.syfjx.20202053
- VernacularTitle:基于UPLC-Q-TOF-MS技术的白花蛇舌草干预结肠炎相关结肠癌小鼠血清代谢组学分析
- Author:
Shu WANG
1
;
Xin WANG
2
;
Ping SUN
2
;
Ying MENG
1
;
Guang-shang CAO
2
;
Peng ZHOU
2
;
Pei-min YANG
2
Author Information
1. Shandong University of Traditional Chinese Medicine （TCM）， Ji'nan 250355，China
2. Affiliated Hospital of Shandong University of TCM， Ji'nan 250011，China
- Publication Type:Research Article
- Keywords:
metabonomics;
Hedyotis diffusa;
differential metabolites;
biomarkers;
metabolic pathways;
colitis associated cancer;
ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry （UPLC-Q-TOF-MS）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(6):94-104
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the intervention of Hedyotis diffusa （HDW） on colitis associated cancer （CAC） model mice and explore its mechanism. Method:The CAC mouse model was established by synergistic action of azoxymethane （AOM） and dextran sulfate sodium （DSS）. The intervention of HDW on CAC mice was evaluated by disease activity index （DAI）， colonic tissue morphology， pathological injury score and tumorigenesis rate. Ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry （UPLC-Q-TOF-MS） and multivariate statistical analysis were used to analyze the metabonomics of mice serum and to explore the mechanism of HDW intervention on CAC. Result:HDW could significantly improve the general condition of CAC mice， decrease DAI， colon gross morphological score， histopathological score and tumorigenesis rate. Compared with the normal group， 38 kinds of differential metabolites were screened in the model group， including 11 potential biomarkers， involving 11 main metabolic pathways. HDW could significantly regulate 9 kinds of differential metabolites ［niacinamide， uridine， 4-pyridoxic acid， LysoPC （18∶0）， LysoPE （0∶0/20∶0）， myo-inositol， purine， sphinganine 1-phosphate and tetradecanedioic acid］ in the model group， including 2 kinds of potential biomarkers （myo-inositol and niacinamide）， and HDW could regulate nicotinate and nicotinamide metabolism and inositol phosphate metabolism. Conclusion:HDW has a therapeutic effect on CAC， which may be achieved by regulation of energy metabolism and glucose metabolism.
