Analysis of Volatile Organic Compounds in Rhizosphere Soil of Rehmannia glutinosa Under Intercropping Zea mays Based on GC-MS Metabonomics
10.13422/j.cnki.syfjx.20210617
- VernacularTitle:基于GC-MS代谢组学分析间作玉米下地黄根际土壤中挥发性有机物特征
- Author:
Li GU
1
;
Xiao-sai TAO
2
;
Feng-qing WANG
3
;
Ming-jie LI
1
;
Chao-fei YANG
3
;
Rui-bin SUN
4
;
Cai-xia XIE
1
;
Zhong-yi ZHANG
1
Author Information
1. Fujian Agriculture and Forestry University，Fuzhou 350002，China
2. Henan University of Chinese Medicine，Zhengzhou 450046，China
3. Henan Agricultural University，Zhengzhou 450002，China
4. Henan Bailiao Huaiyao Science and Technology Development Co. Ltd.，Jiaozuo 454000，China
- Publication Type:Research Article
- Keywords:
intercropping pattern;
Rehmannia glutinosa;
Zea mays;
rhizosphere soil;
gas chromatography-mass spectrometry （GC-MS）;
volatile organic compounds
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(10):102-108
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the composition characteristics of rhizosphere soil under Rehmannia glutinosa-Zea mays intercropping model，and screen out special signal substances in rhizosphere soil of R. glutinosa under intercropping Z. mays， so as to provide the basis for the study of allelopathic substances in continuous cropping obstacle of R. glutinosa. Method:In this experiment，rhizosphere soils of R. glutinosa under Z. mays intercropping and R. glutinosa single cropping models in July，August，September and October were taken as the research objects， and the volatile organic compounds in ethyl acetate fraction were analyzed by gas chromatography-mass spectrometry （GC-MS）. Principal component analysis （PCA）， hierachical cluster analysis （HCA） and orthogonal partial least squares discriminant analysis （OPLS-DA） analysis were performed on the data by SIMCA 14.1 to screen out potential differences in volatile organic compounds between the two models. Result:The types of volatile organic compounds in intercropping and single cropping models were mainly hydrocarbons， alcohols， esters， ketones， amides， acids and other substances. Specifically， the average relative contents of hydrocarbons，esters and amides in intercropping model were 58.46%，32.15% and 5.42% respectively，while the relative contents of hydrocarbons，esters and amides in single cropping model were 37.27%，36.11% and 21.13%. The results of PCA and HCA showed that the characteristics of volatile organic compounds in the ethyl acetate fraction of rhizosphere soil under intercropping and single cropping models could be clearly divided into two categories，the screening results of potential differential components based on OPLS-DA analysis indicated that various components， such as dibutyl phthalate，（Z）-9-oleamide，β-caryophyllene，dioctyl iso-phthalate， phthalate （2-propylamyl） diester， n-hexadecane，octodecane， n-heneicosane， were screened from rhizosphere soil under the two models. Conclusion:The R. glutinosa-Z. mays intercropping model has certain effects on the volatile organic compounds in the rhizosphere soil of R. glutinosa，and the effect of the selected components on the growth and quality characteristics of R. glutinosa still need to be further studied.
