Metabolomics Analysis of Chloriti Lapis on Brain Tissue of PTZ-kindled Epileptic Rats
10.13422/j.cnki.syfjx.20210150
- VernacularTitle:矿物药青礞石对PTZ点燃癫痫大鼠影响的脑组织代谢组学分析
- Author:
Sheng-jin LIU
1
;
Lu-ting WU
1
;
Yu-lu MA
1
;
Fang FANG
1
;
Wen-guo YANG
1
;
Chen-xiao SHAN
1
;
Yong BIAN
1
;
Hui YAN
1
;
Zhi-jie ZHANG
2
;
Wu-li-ji AO
3
;
Jin-ao DUAN
1
Author Information
1. Key Laboratory of Chinese Medicinal Resources Recycling Utilization， National Administration of Traditional Chinese Medicine，National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine， Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization， School of Pharmacy，Nanjing University of Chinese Medicine，Nanjing 210023，China
2. Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100700，China
3. Inner Mongolia University For Nationalities，Tongliao 028000，China
- Publication Type:Research Article
- Keywords:
mineral Chinese medicines;
Chloriti Lapis;
epilepsy;
pentylenetetrazol （PTZ）;
brain tissue;
metabonomics;
mechanism of action
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(10):76-84
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the possible mechanism of Chloriti Lapis in the treatment of epilepsy by the metabonomics of brain tissue in pentylenetetrazol （PTZ）-kindled epileptic rats treated with Chloriti Lapis. Method:The epileptic animal model in rats was established by PTZ kindling， and the rats were divided into the control group， model group， carbamazepine group and Chloriti Lapis group. The brain tissue samples were detected by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry（UPLC/Q-TOF-MS）， and the experimental results were statistically analyzed by partial least squares-discriminant analysis （PLS-DA） and SPSS 18.0. Result:The metabolic fingerprints and metabolic profiles of the rat brain tissue were established， which showed that the metabolic profiles of each group had changed significantly and could be separated well among the groups. Moreover， the Chloriti Lapis group had a tendency to be closer to the control group than the carbamazepine group. Seven differential metabolites were screened， including phosphatidylserine （PS） （18∶0/18∶0）， L-glutamic acid， docosahexaenoyl ethanolamide， arachidonic acid， glucosylsphingosine， cholestane-3，7，12，24，25-pentol and lysophosphatidylcholine （LysoPC） （P-18∶0）. Except for docosahexaenoyl ethanolamide and LysoPC （P-18∶0）， Chloriti Lapis had significant intervening and regulating effects on the other five differential metabolites. There were 12 possible metabolic pathways that affected the metabolic disorder of PTZ-kindled rats， and 3 important metabolic pathways （pathway impact>0.1）， namely， D-glutamine and D-glutamate metabolism， alanine， aspartate and glutamate metabolism， and arachidonic acid metabolism， among which D-glutamine and D-glutamate metabolism was the most important metabolic pathways. Conclusion:From this point of view， Chloriti Lapis has a clear intervention effect on PTZ-kindled epileptic rats， which may be related to the intervention of the above differential metabolite contents and related metabolic pathways. It can reduce the toxic effect of excitatory neurotransmitters on neurons in brain tissue and inhibit the development of inflammation in brain tissue， so as to maintain the biological function of brain cells and slow down the occurrence of epilepsy.
