Intervention Mechanism of Yishen Huayu Prescription on Glomerular Podocyte Injury in Diabetic Nephropathy Rats Based on EMT Regulated by Wnt/β-catenin Pathway
10.13422/j.cnki.syfjx.20210801
- VernacularTitle:基于Wnt/β-catenin调控EMT探讨益肾化瘀方对糖尿病肾病大鼠肾小球足细胞损伤的干预机制
- Author:
Wei-guo SONG
1
;
Lin PENG
1
;
Wen-wen WU
1
;
Chi-heng PI
2
Author Information
1. Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine（TCM），Nanchang 330006，China
2. PI Chi-heng Famous Chinese Medicine Studio，Affiliated Hospital of Jiangxi University of TCM，Nanchang 330006，China
- Publication Type:Research Article
- Keywords:
diabetic nephropathy;
glomerular podocyte injury;
Wnt/β-catenin pathway;
epithelialmesenchymal transition （EMT）;
Yishen Huayu prescription;
intervention mechanism
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(10):44-50
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the intervention mechanism of Yishen Huayu prescription on glomerular podocyte injury in diabetic nephropathy （DN） rats based on epithelialmesenchymal transition （EMT） regulated by Wnt/β-catenin pathway. Method:The 60 SD rats were divided into control group， model group， Wnt-C59 group （0.03 g·kg-1 Wnt/β-catenin pathway inhibitor）， low-dose group （8 g·kg-1）， medium-dose group （16 g·kg-1） and high-dose group （32 g·kg-1）. After 12 weeks， various indexes ， including general signs， serum creatinine （SCr）， blood urea nitrogen （BUN）， renal index， urinary protein， blood glucose， renal pathological changes， podocyte and expressions of glomerular basement membrane injury and podocyte injury related proteins ［nephrin， synaptopodin］， Wnt/β-catenin pathway related proteins （Wnt1， β-catenin）， podocyte EMT related protein ［α-smooth muscle actin （α-SMA）， E-cadherin］， were compared between groups. Result:Compared with the control group， the renal tissue in the model group showed significant pathological changes， including diffuse thickening of glomerular mesangial matrix and severe foot process fusion， and a significant increase in SCr， BUN， renal indexes， urinary protein， blood glucose， Wnt1， β-catenin， and α-SMA expression levels （P<0.05） as well as a significant decrease in nephrin， synaptopodin and E-cadherin expression levels（P<0.05）. Compared with model group， SCr， BUN， renal index， urinary protein， blood glucose， Wnt1， β-catenin， and α-SMA expression levels in each intervention group significantly decreased （P<0.05）， while the expression levels of nephrin， synaptopodin and E-cadherin significantly increased （P<0.05）. Among intervention groups， the improvement of above indexes in high-dose Yishen Huayu prescription group was the most obvious （P<0.05）， which was similar to the effect in Wnt-C59 group. Conclusion:Yishen Huayu prescription prevents podocyte EMT by inhibiting Wnt/β-catenin pathway， thereby repairing glomerular podocyte injury in rats with diabetic nephropathy.
