Analysis of Chemical Constituents in Jiechangyan Qixiao Granules Based on UPLC-Q-TOF/MSE
10.13422/j.cnki.syfjx.20202356
- VernacularTitle:结肠炎奇效颗粒中化学成分的UPLC-Q-TOF/MSE分析
- Author:
Yu-jing SUN
1
;
Zhi-peng HUO
2
;
Yu WANG
2
;
Rui-ming LI
1
;
Min-jian QIN
1
;
Yi HE
2
Author Information
1. School of Traditional Chinese Pharmacy，China Pharmaceutical University，Nanjing 210009，China
2. Development Center of Modern Chinese Medicine，Research Institute of Tasly Holding Group Co. Ltd.，Tianjin 300410，China
- Publication Type:Research Article
- Keywords:
Jiechangyan Qixiao granules;
ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry （UPLC-Q-TOF/MSE）;
material basis;
flavonoids;
iridoid glycosides;
triterpenes;
Chinese herbal compound
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(9):157-167
- CountryChina
- Language:Chinese
-
Abstract:
Objective:In order to systematically clarify the chemical composition of Jiechangyan Qixiao granules， the main chemical components in this preparation were rapidly identified and assigned by ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry （UPLC-Q-TOF/MSE）. Method:ACQUITY UPLC BEH C18 column （2.1 mm×100 mm， 1.8 μm） was employed for UPLC analysis with the mobile phase of 0.1% formic acid aqueous solution （A）-acetonitrile （B） for gradient elution （0-2 min， 5%B； 2-16 min， 5%-21%B； 16-30 min， 21%-95%B； 30-33 min， 95%B； 33-34 min， 95%-5%B； 34-37 min， 5%B）. The flow rate was 0.3 mL·min-1， the column temperature was 30 ℃， and the volume of sample injection was 2 μL. Electrospray ionization （ESI） was applied for scanning under positive and negative ion modes with the scanning range of m/z 60-1 200. MSE mode was used to collect mass spectral data. The ion peaks were identified by comparing with the information of control substances， literature references and self-built database. Result:A total of 102 chemical components were separated and identified in Jiechangyan Qixiao granules， including organic acids， flavonoids and its glycosides， triterpenes， phenylethanoid glycosides， tannins， iridoid glycosides and other components， among which flavonoids and its glycosides were from Drynariae Rhizoma and Crataegi Fructus， phenylethanoid glycosides and iridoid glycosides were from Plantaginis Semen， triterpenoids and tannins were from Crataegi Fructus and Chebulae Fructus. Among the identified chemical constituents， there were 28 from Drynariae Rhizoma， 31 from Plantaginis Semen， 53 from Chebulae Fructus and 58 ingredients from Crataegi Fructus. Conclusion:The established UPLC-Q-TOF/MSE can comprehensively and rapidly analyze the chemical constituents in Jiechangyan Qixiao granules， and preliminarily elucidates the chemical composition profile of this granules， which can lay a foundation for further research on the pharmacodynamic material basis and quality control of Jiechangyan Qixiao granules.
