Effect of Removing Core on Quality of Phyllanthi Fructus
10.13422/j.cnki.syfjx.20210147
- VernacularTitle:去核对余甘子药材质量的影响
- Author:
Chuan-hong LUO
1
;
Sheng-jie HUANG
1
;
Qi-qi HU
1
;
Peng TAN
2
;
Hua-mei GOU
1
;
Xi-chuan WEI
1
;
Hao-zhou HUANG
1
;
San-hu FAN
3
;
Jun-zhi LIN
4
;
Ding-kun ZHANG
1
;
Li HAN
1
Author Information
1. School of Pharmacy，State Key Laboratory of Characteristic Chinese Drug Resources in Southwest China，Chengdu University of Traditional Chinese Medicine（TCM），Chengdu 611137，China
2. State Key Laboratory of Biological Evaluation of TCM Quality，National Administration of TCM， Sichuan Academy of Chinese Medicine Sciences，Chengdu 610041，China
3. Sanajon Pharmaceutical Group，Chengdu 610000，China
4. Teaching Hospital of Chengdu University of TCM，Chengdu 610072，China
- Publication Type:Research Article
- Keywords:
Phyllanthi Fructus;
removing core;
high performance liquid chromatography （HPLC）;
antibacterial activity;
antioxidant activity;
Chinese medicinal materials
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(9):147-156
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The differences of chemical compositions and pharmacological activities between the core and pulp of Phyllanthi Fructus were investigated by chemical analysis and in vitro test to explore the effect of the core on the quality of this medicinal material. Method:Literature， medicinal material standards and market research on the appearance of Phyllanthi Fructus were conducted based on existing databases. Ultra-high performance liquid chromatography-quadrupole-electrostatic field orbital trap high resolution mass spectrometry （UPLC-Q-Orbitrap HRMS） was used to identify the constituents of the core and pulp. The analysis was performed on Thermo Scientific Accucore C18 column （2.1 mm×100 mm， 2.6 μm） with the mobile phase of 0.1% formic acid aqueous solution （A）-methanol （B） for gradient elution （0-25 min， 5%B； 25-30 min， 5%-95%B； 30-35 min， 95%-5%B）， the flow rate was 0.2 mL·min-1， heating electrospray ionization （HESI） was adopted with positive and negative ion modes， and the scanning range was m/z 100-1 500. High performance liquid chromatography （HPLC） was used to determine the contents of gallic acid， corilagin， chebulagic acid and ellagic acid in the core and pulp of Phyllanthi Fructus. Analysis was performed on Welchrom C18 column （4.6 mm×250 mm， 5 μm） with mobile phase of methanol （A）-0.05% phosphoric acid aqueous solution （B） for gradient elution （0-6 min， 5%A； 6-15 min， 5%-7%A； 15-20 min， 7%-15%A； 20-25 min， 15%-21%A； 25-31 min， 21%-22%A； 31-41 min， 22%A； 41-47 min， 22%-28%A； 47-51 min， 28%-32%A； 51-57 min， 32%-38%A； 57-70 min， 38%-45%A； 70-80 min， 45%-65%A； 80-85 min， 65%-5%A）， the detection wavelength was set at 270 nm. The antibacterial effects of the core and pulp of Phyllanthi Fructus on Escherichia coli and Staphylococcus aureus were investigated by filter paper method， and their antioxidant activities were compared by 1，1-diphenyl-2-picrylhydrazyl （DPPH） free radical scavenging assay. Result:A total of 47 compounds were identified in the core and pulp of Phyllanthi Fructus， mainly including tannins， flavonoids， phenolic acids， fatty acids， amino acids， organic acids， saccharides and glycosides， most of which were concentrated in the pulp， and the fatty acids in the core accounted for a higher proportion. The contents of gallic acid， corilagin， chebulagic acid， ellagic acid and other phenolic compounds in the pulp of 20 batches of Phyllanthi Fructus were much higher than those in the core. The results of antibacterial test showed that the core of Phyllanthi Fructus with different concentrations had no antimicrobial effect. The DPPH radical scavenging test showed that the antioxidant activity of the core ［half-inhibitory concentration （IC50）=199.632 mg·L-1］ was much less than that of the pulp （IC50=12.688 mg·L-1）. Conclusion:From the perspectives of polyphenol content， antibacterial and antioxidant activities， it is scientific to use Phyllanthi Fructus pulp in ancient and modern times， which may be to remove the secondary parts of Phyllanthi Fructus， so as to enhance the actual utilization rate and therapeutic effect of medicinal materials. In view of the large proportion of the core of Phyllanthi Fructus and its high content of fatty acids and other components， whether or not to use it remains to be further studied in clinical application.
