Study on Sub-minimal Inhibitory Concentration of Tanreqing Injection in Inhibiting Hemolytic Activity of Staphylococcus aureus α-hemolysin
10.13422/j.cnki.syfjx.20210605
- VernacularTitle:亚抑菌浓度痰热清注射液抑制金黄色葡萄球菌α-溶血素溶血活力
- Author:
Qian TONG
1
;
Yi WANG
2
;
Jin-he ZHOU
3
;
Kai-yu CUI
2
;
Zhuo MA
1
;
Wei-feng YANG
2
Author Information
1. Hubei University of Technology，Wuhan 430068，China
2. Experimental Research Center，China Academy of Chinese Medical Sciences， Beijing 100700， China
3. Jilin Agricultural University，Changchun 130118，China
- Publication Type:Research Article
- Keywords:
Staphylococcus aureus;
α-hemolysin;
Tanreqing injection;
sub-minimal inhibitory concentration
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(9):86-93
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study whether Tanreqing injection （TRQ） can alleviate the body injury in the process of infection by inhibiting the production and release of α-hemolysin of Staphylococcus aureus under sub-minimal inhibitory concentration， and to provide experimental basis for better guidance of clinical medication. Method:The effects of TRQ on the minimum inhibitory concentration （MIC） and bacterial growth of S.aureus were determined firstly by microplate method and time-growth curve. The different sub-minimal inhibitory concentrations of TRQ were co-cultured with bacteria or bacterial supernatants， and then co-incubated with defibrillated rabbit blood to detect the inhibitory and neutralizing effects of TRQ on S.aureus α-hemolysin. Cell counting kit-8 （CCK-8） cell viability assay was used to detect the protective effect of TRQ on S. aureus-mediated damage to human alveolar epithelial cells （A549）. Real-time fluorescence quantitative polymerase chain reaction （Real-time PCR） was used to detect the effect of sub-minimal inhibitory concentration of TRQ on the mRNA expression of S.aureus α-hemolysin regulatory genes hla and agrA. Result:The MIC of TRQ to S.aureus was 1/8 of the stock solution， and the sub-minimal inhibitory concentration （1/64MIC-1/16MIC） TRQ used in this study did not affect the growth of bacteria. 1/64MIC-1/16 MIC TRQ had the effect of inhibiting and neutralizing the hemolytic activity of α-hemolysin， with a protective effect on S.aureus supernatant-mediated A549 cell damage， and its inhibitory effect on α-hemolysin was closely related to the inhibition of hla and agrA mRNA expression. Conclusion:The sub-minimal inhibitory concentration TRQ can inhibit and neutralize the hemolytic activity of α-hemolysin of S.aureus， with a protective effect on A549 cell damage mediated by S.aureus infection， and its mechanism of inhibiting α-hemolysin is closely related to the interference with agr regulatory system.
