Da Chaihutang Protects Liver Mitochondrial Function of Nutritionally Obese Rats via CREB/PGC-1α Pathway
10.13422/j.cnki.syfjx.20210601
- VernacularTitle:大柴胡汤通过CREB/PGC-1α通路保护营养型肥胖大鼠肝脏粒体功能
- Author:
Long-chun LI
1
;
Xiao-ye LI
2
;
Xiao-hua DU
3
;
Tie-qu CHAI
1
Author Information
1. Clinical Medical College of Acupuncture，Moxibustion and Rehabilitation，Guangzhou University of Chinese Medicine，Guangzhou 510405，China
2. Maternal and Child Health Hospital of Bao'an District，Shenzhen 518136，China
3. Graduate School of Guangxi University of Chinese Medicine，Nanning 530200，China
- Publication Type:Research Article
- Keywords:
Da Chaihutang;
nutritionally obese rat;
mitochondria;
cAMP-response element binding protein （CREB）/peroxisome proliferator-activated receptor-gamma coactivator-1 alpha （PGC-1α） pathway
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(9):26-31
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effects of Da Chaihutang on Cyclic adenosine monophosphate （cAMP）-response element binding protein （CREB）/peroxisome proliferator-activated receptor-gamma coactivator-1 alpha （PGC-1α） pathway in nutritionally obese rats and the protective mechanism on liver mitochondria. Method:A total of 120 8-week-old male SD rats were randomly divided into a control group （n=20） and an experimental group （n=100）. The rats in the control group were fed on a normal diet， while those in the experimental group were administered with a high-fat feed. Successfully modeled rats were randomly divided into a model group， a positive drug （metformin） group， and low-， medium- and high-dose Da Chaihutang groups （4.25， 8.5， and 17 g∙kg-1， respectively）， with 20 rats in each group. After treatment with Da Chaihutang， the body weight， Lee's index， liver mitochondrial membrane potential and mitochondrial ultrastructure， PGC-1α expression and CREB phosphorylation of each group were measured and compared. Result:Compared with the control group， the model group showed increased body weight and Lee's index （P<0.01）， whereas decreased mitochondrial membrane potential， PGC-1α expression， and CREB phosphorylation level （P<0.01）. As compared with the model group， Da Chaihutang significantly reduced the body weight and Lee's index of obese rats （P<0.05， P<0.01）， enhanced liver mitochondrial membrane potential （P<0.05， P<0.01） to protect the integrity of mitochondrial structure， up-regulated PGC-1α expression and promoted CREB phosphorylation （P<0.05， P<0.01）. Conclusion:Da Chaihutang protects the structure and function of mitochondria and inhibits weight gain in obese rats by activating the CREB/PGC-1α pathway.
