Diversity and Anti-Dermatophyte Activities of Endophytic Fungi from Datura metel
10.13422/j.cnki.syfjx.20210714
- VernacularTitle:白花曼陀罗内生真菌多样性及抗皮肤真菌活性分析
- Author:
Mei-ru FENG
1
;
Wen-guang SHI
1
;
Jia-qi LI
1
;
Dan-ting CHEN
1
;
Ying-hao FU
1
;
Zu-jun DENG
1
Author Information
1. Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances，School of Life Sciences and Biopharmaceutics，Guangdong Pharmaceutical University，Guangzhou 510006，China
- Publication Type:Research Article
- Keywords:
Datura metel;
endophytic fungi;
diversity;
anti-dermatophyte activities
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(11):187-194
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the taxonomic structure and diversity of endophytic fungi from Datura metel and screen the strains with anti-dermatophyte activities， so as to provide resources for the development of new lead compounds against dermatomycosis. Method:Endophytic fungi were isolated from the roots， stems and leaves of D. metel after tissue block incubation and then identified by morphological analysis and rDNA-internal transcribed spacer（ITS） sequencing. Their anti-dermatophyte activities were detected by agar diffusion assay. Result:A total of 292 strains of endophytic fungi were isolated from D. metel， belonging to 34 genera， with Fusarium （72.97%） in roots， Fusarium （37.25%） and Plectosphaerella （28.43%） in stems， and Colletotrichum （39.66%） in leaves as the dominant species. The isolation rate （89.23%）， colonization rate （84.62%）， and diversity index （1.82） of endophytic fungi in leaves were significantly higher than those in roots （70.48%， 70.48% and 1.23） and stems （69.39%，68.03% and 1.64）. The determination of anti-dermatophyte activities of 35 endophytic fungal fermented filtrates showed that the strains exhibiting inhibitory activities against Microsporum canis， Trichosporon mucoides， Trichophyton rubrum and Candida albicans accounted for 97.14%， 71.43%， 45.71%， and 25.71%， respectively. Among them， six strains （17.14%）， namely Fusarium sp. R1， Penicillium sp. R5， Aspergillus sp. R7， Metarhizium sp. S18， Diaporthe sp. S19， and Glomerella sp. L57， all inhibited the four types of cutaneous fungal pathogens. Conclusion:The endophytic fungi in D. metel are diverse， and the proportion of endophytic fungi possessing anti-dermatophyte activities is high， allowing them to serve as potential resources for the development of new anti-dermatophyte agents.
