Fusarium Wilt Changes Microbial Community Structure in Rhizosphere Soil of Chrysanthemum morifolium
10.13422/j.cnki.syfjx.20210713
- VernacularTitle:枯萎病对菊花根际土壤微生物群落结构的影响
- Author:
Qiao-huan CHEN
1
;
Yu-huan MIAO
1
;
Tie-lin WANG
2
;
Lan-ping GUO
1
;
Da-hui LIU
1
Author Information
1. Pharmacy Faculty，Hubei University of Chinese Medicine， Wuhan 430065，China
2. National Resource Center for Chinese Materia Medica，China Academy of Chinese Medical Sciences， Beijing 100700，China
- Publication Type:Research Article
- Keywords:
Chrysanthemum morifolium;
Fusarium wilt;
rhizosphere soil;
microbial community;
high-throughput sequencing
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(11):180-186
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the differences in rhizosphere microbial community structure between Fusarium wilt-infected and healthy Chrysanthemum morifolium plants. Method:The rhizosphere soils of diseased and healthy C. morifolium plants were sampled and subjected to high-throughput 16S ribosomal DNA （rDNA） and internal transcribed spacer （ITS） sequencing， to identify the microbial community structure including bacteria and fungi. Result:Fusarium wilt reduced the bacterial abundance and diversity but had no significant effect on fungal alpha-diversity.The proportions of Acidobacteria， Gemmatimonadetes， and Nitrospirae in rhizosphere soil of healthy C.morifolium plants were higher than those of diseased plants， while the proportions of Proteobacteria and Bacteroidetes were lower（P<0.05）. Fusarium fungi accounted for 27.49%， 14.53%， and 11.94% in diseased plants whereas 0.47%， 1.01%， and 0.67% in healthy plants.Pathogenic bacteria Pectobacterium and Dickeya were enriched in rhizosphere soil of diseased plants. The abundances of nitrifying， detoxifying， and photosynthetic bacteria in rhizosphere soil of healthy plants were higher than those of diseased plants. Conclusion:Fusarium wilt reduces the bacterial richness and diversity and triggers the enrichment of massive Fusarium fungi， Pectobacterium， and Dickeya. The proportion of beneficial bacteria in rhizosphere soil of healthy plants is significantly higher than that of diseased plants.
