Effect of Chloriti Lapis on Metal Elements in Brain and Plasma of PTZ-kindled Epileptic Rats
10.13422/j.cnki.syfjx.20210178
- VernacularTitle:矿物药青礞石对PTZ点燃癫痫大鼠脑组织、血浆中金属元素的影响
- Author:
Sheng-jin LIU
1
;
Lu-ting WU
1
;
Yu-lu MA
1
;
Fang FANG
1
;
Yong BIAN
1
;
Chen-xiao SHAN
1
;
Wen-guo YANG
1
;
Hui YAN
1
;
Zhi-jie ZHANG
2
;
Wu-li-ji AO
3
;
Jin-ao DUAN
1
Author Information
1. Key Laboratory of Chinese Medicinal Resources Recycling Utilization，National Administration of Traditional Chinese Medicine，National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine， Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization， School of Pharmacy，Nanjing University of Chinese Medicine，Nanjing 210023，China
2. Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100700，China
3. Inner Mongolia University for Nationalities，Tongliao 028000，China
- Publication Type:Research Article
- Keywords:
mineral medicines;
Chloriti Lapis;
epilepsy;
pentylenetetrazol （PTZ）;
brain tissue;
plasma;
metal elements;
material basis
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(11):130-138
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of Chloriti Lapis on metal elements in brain tissue and plasma of epileptic rats kindled by pentylenetetrazol （PTZ）， and to explore the possible material basis of Chloriti Lapis. Method:PTZ kindling method was used to establish epileptic rat model. Inductively coupled plasma mass spectrometry （ICP-MS） and inductively coupled plasma optical emission spectrometer （ICP-OES） were used to determine the contents of metal elements in brain tissue and plasma of the blank group， model group， carbamazepine group （0.1 g·kg-1） and Chloriti Lapis group （2 g·kg-1）. The data were statistically analyzed by SPSS 18.0 software. Result:Compared with the blank group， the contents of Sr， Sb and Ba in brain tissue of rats in the model group were significantly increased （P<0.05， P<0.01）， while the contents of Zn， Fe， Cu， K， Li， Co， Sn and Pb were significantly decreased （P<0.05， P<0.01）. Compared with the model group， the contents of Zn， Fe， K， Li， Co， As and Pb in brain tissue of rats in the Chloriti Lapis group were obviously increased （P<0.05， P<0.01）， while the contents of Sr and Sb were significantly decreased （P<0.01）. These results showed that Chloriti Lapis had positive effect on the regulation of the content of metal elements in rat brain tissue to normal level， the intervention effect was clear， and the overall effect was better than that of carbamazepine group. The determination of 21 metal elements in plasma showed that compared with the blank group， the levels of K， Sr and Cd in the model group were significantly increased （P<0.05）， and the contents of Li， Al， Ti and Cr were significantly decreased （P<0.05）. Compared with the model group， the contents of Ca， K， Li， Al and V in the Chloriti Lapis group were obviously increased （P<0.05， P<0.01）， and the contents of Fe， Ti， Sr and Cd were significantly decreased （P<0.05，P<0.01）. The correlation analysis of metal elements among the groups showed that there were 17 pairs of elements had positively correlation in the brain tissue of rats， 2 pairs of elements had significant negative correlation. In the plasma of rats， 8 pairs of elements had significant positive correlation and 6 pairs of elements had significant negative correlation. Conclusion:The metal element groups represented by Zn， Fe， K， Li， Co， As， Pb， Sr， Sb， Ca， Al， V， Ti and Cd may be the effective material basis for Chloriti Lapis to interfere PTZ-kindled epileptic model rats， which may be related to the influence of these metal element groups on the release of neurotransmitters and the electrical balance of neurons， the regulation of abnormal synchronous discharge induced by Na+， K+， Ca2+ channel disorders and intervention of metabolism pathways in brain tissue related to epilepsy. It can make the excitatory and inhibitory activities restrain each other， and finally reach the normal physiological state of neurons and cells. The intervention effect of Chloriti Lapis group was better than that of carbamazepine group.
