Effects of Dimethyl Sulfoxide and Ethanol on Adventitious Root Growth of Panax quinquefolium and Ginsenosides
10.13422/j.cnki.syfjx.20210811
- VernacularTitle:二甲基亚砜和乙醇处理对西洋参不定根表型和人参皂苷含量的影响
- Author:
Jin HU
1
;
Hong-hong JIAO
2
;
Tie-gui NAN
2
;
Yu-yang ZHAO
2
;
Jun-hui ZHOU
2
;
Yuan YUAN
2
Author Information
1. School of Pharmacy， Anhui University of Chinese Medicine，Hefei 230012，China
2. State Key Laboratory Breeding Base of Dao-di Herbs，National Resource Center for Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100700，China
- Publication Type:Research Article
- Keywords:
Panax quinquefolium;
adventitious roots;
dimethyl sulfoxide （DMSO）;
ethanol;
ginsenoside
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(12):141-146
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To screen out the suitable nonpolar molecular cosolvent and concentration with adventitious root phenotype and ginsenoside content in the controlled experiment as the evaluation indexes， so as to lay a solid foundation for exploring the causes for good shape and high quality of Panax quinquefolium. Method:After being treated with different concentrations of dimethyl sulfoxide （DMSO） and ethanol， the adventitious roots were scanned using a panoramic scanner， and the resulting images were used for measuring the branch number and average diameter by WinRHIZO Pro 2016， Synbiosis ProtoCol 3 colony counter， Image J， and SmartRoot. The contents of ginsenosides Rg1， Rb1， and Re were determined by ultra-performance liquid chromatography tandem mass spectrometry （UPLC-MS/MS）. Result:Compared with the blank control， the 0.1% DMSO and 75% ethanol made the adventitious root phenotype and ginsenoside contents significantly changed. Specifically， the branch number and average diameter were significantly reduced. The ginsenoside Rg1 in the adventitious roots decreased after 0.1% DMSO treatment， whereas the ginsenosides Rg1 and Re increased after 75% ethanol treatment. The adventitious root phenotype and ginsenoside contents in the 0.1% DMSO treatment group were not significantly different from those in the control group. Conclusion:The 0.01% DMSO does not affect the adventitious root growth of P. quinquefolium and is insoluble in water， enabling it to be considered as a suitable nonpolar molecular cosolvent for future research on the genetic causes for the good shape and high quality of P. quinquefolium.
