Effect of Curdione on MDA-MB-231 Cell Cycle and Apoptosis
10.13422/j.cnki.syfjx.20211298
- VernacularTitle:莪术二酮对MDA-MB-231细胞周期和凋亡的影响
- Author:
Kai-yuan ZHANG
1
;
Ling-ling LYU
1
;
Jing-xian CHEN
1
;
Jia-yue XU
1
;
Qiong LI
1
;
Yuan WU
1
;
Lan ZHENG
1
Author Information
1. Ruijin Hospital，Shanghai Jiaotong University School of Medicine，Shanghai 200025，China
- Publication Type:Research Article
- Keywords:
curdione;
triple negative breast cancer;
MDA-MB-231 cells;
cell cycle;
apoptosis
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(12):74-81
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of curdione on the proliferation， apoptosis and cell cycle of triple negative breast cancer cell line MDA-MB-231. Method:MDA-MB-231 cells were cultured in vitro with capecitabine （positive control） and curdione at different concentrations （125， 250， 500， 1 000， and 2 000 μmol·L-1）， respectively， for detecting their viability using the cell counting kit-8 （CCK-8） at 24 and 48 h. Three effective inhibitory concentrations （250， 500， and 1 000 μmol·L-1） against cell proliferation were selected for subsequent experiments. The effect of curdione on cell cycle was determined by flow cytometry combined with propidium iodide （PI） staining. After the set-up of high-concentration （2 000 μmol·L-1） group， the effect of curdione on cell mitochondrial membrane potential was measured by JC-1（5，5，6，6-tetrachloro-1，1，3，3-tetraethylbenzimidazolylcarbocyanine iodide） staining， followed by the detection of cell apoptosis by flow cytometry combined with Annexin V-FITC/PI double staining. The changes in cell cycle status and apoptosis-related protein expression following curdione intervention were assayed by Western blot. Result:Compared with the blank control， curdione at 250， 500， 1 000， and 2 000 μmol·L-1 significantly inhibited the proliferation of MDA-MB-231 cells （P<0.01）， exhibiting a concentration- and time-response relationship. The half maximal inhibitory concentration （IC50） values at 24 and 48 h were 1 607 and 1 401 μmol·L-1， respectively. Curdione at 250， 500， and 1 000 μmol·L-1 arrested cells in G1 phase. Curdione at 250 μmol·L-1 had no effect on cell mitochondrial membrane potential， which， however， declined significantly in the 500， 1 000， and 2 000 μmol·L-1 groups （P<0.05， P<0.01）. Curdione at 250， 500， and 1 000 μmol·L-1 obviously increased the proportion of apoptotic cells （P<0.05， P<0.01）. Curdione at each concentration elevated the Bcl-2-associated X protein （Bax）/B-cell lymphoma 2 （Bcl-2） ratio （P<0.05， P<0.01）， but did not change the cysteinyl aspartate-specific protease-3 （Caspase-3） expression. The protein expression levels of Caspase-9， cleaved Caspase-9， cleaved Caspase-3， p53， and p21 were up-regulated （P<0.05）. Conclusion:A certain concentration of curdione inhibits the proliferation of MDA-MB-231 cells， which may be related to its efficacy in arresting cell cycle and inducing apoptosis.
