Study on Mechanism of Quercetin in Heart Failure Based on Network Pharmacology and Experimental Validation
10.13422/j.cnki.syfjx.20210919
- VernacularTitle:基于网络药理学和实验验证探讨槲皮素治疗心力衰竭的分子机制
- Author:
Liang ZHANG
1
;
Shuang-rong YANG
1
;
Shu-ying LI
1
;
Xiu-fen YANG
1
;
Hao LIN
2
;
Yuan-zheng MA
1
;
Shan-liang LI
1
Author Information
1. Guangxi University of Chinese Medicine，Nanning 530022，China
2. Food and Drug Control Institute of Guangxi Zhuang Autonomous Region，Nanning 530022，China
- Publication Type:Research Article
- Keywords:
quercetin;
network pharmacology;
heart failure;
target prediction;
cell validation
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(15):156-165
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To predict the therapeutic targets and related signaling pathways of quercetin in the treatment of heart failure （HF） by network pharmacology and molecular docking methods，and further clarify its mechanisms through in vitro cell model. Method:The pharmacological targets of quercetin were obtained by SwissTargetPrediction and Targetnet databases； the heart failure related targets were obtained by Online Mendelian Inheritance in Man（OMIM），GeneCards and Therapeutic Target Database（TTD） databases； the protein-protein interaction（PPI） network was analyzed by STRING database（Search Tool for Recurring Instances of Neighbouring Genes），and the PPI network diagram of quercetin for heart failure target was established. Cytoscape 3.7.2 software was used for analyzing and screening the anti-heart failure network nodes of quercetin，and the obtained targets were enriched with gene ontology （GO） and Kyoto Encyclopedia of Genes and Genomes （KEGG） analysis by DAVID database. In order to explore the mechanism of quercetin in the treatment of heart failure，we used cell model to verify the function in heart failure treatment. Results:The predicted results showed that there were 23 targets for the treatment of heart failure，such as Matrix Metallopeptidase-9（MMP-9），androgen receptor（AR），coagulation factor 2（F2），insulin like growth factor 1 receptor（IGF1R），epidermal growth factor receptor（EGFR），janus kinase-2（JAK2），cytochrome P450 family 19 subfamily A member 1（CYP19A1），estrogen receptor-1（ESR1），tumor necrosis factor（TNF），protein tyrosine phosphatase receptor type C（PTPRC） and cytochrome P450 family 17 subfamily A member 1（CYP17A1） etc. The results suggest that quercetin may play a role in the treatment of heart failure by intervening in the physiological processes of cardiovascular cell proliferation and metabolism，regulating hypoxia-inducible factor 1 （HIF-1）signaling pathway and steroid hormone biosynthesis. Conclusion:Quercetin has the characteristics of multi-target，multi-channel and multi-channel in the treatment of heart failure. It may play a role in the treatment of heart failure by regulating MMP-9，EGFR and other key genes，participating in the biological process of cardiac and vascular cell proliferation and metabolism.
