Identification of Chemical Constituents in Coptis chinensis Inflorescence by UPLC-Q-Orbitrap HRMS
10.13422/j.cnki.syfjx.20210860
- VernacularTitle:黄连花薹化学成分的UPLC-Q-Orbitrap HRMS鉴定
- Author:
Ye ZHANG
1
;
Qi DENG
2
;
Min WEI
1
;
Xu ZHANG
1
Author Information
1. School of Pharmacy，Chengdu University of Traditional Chinese Medicine，Chengdu 611137，China
2. Sichuan Kanghe Dingsheng Grand Health Industry Group Co. Ltd.，Chengdu 610000，Sichuan
- Publication Type:Research Article
- Keywords:
ultra-high performance liquid chromatography coupled with quadrupole-orbitrap high resolution mass spectrometry （UPLC-Q-Orbitrap HRMS）;
Coptis chinensis inflorescence;
chemical constituents;
alkaloids;
flavonoids;
phenylpropanoids;
organic acids
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(15):91-99
- CountryChina
- Language:Chinese
-
Abstract:
Objective:An ultra-high performance liquid chromatography coupled with quadrupole-orbitrap high resolution mass spectrometry （UPLC-Q-Orbitrap HRMS） was developed to analyze and identify the chemical constituents in Coptis chinensis inflorescence. Method:The chromatographic separation was performed on ACQUITY UPLC BEH C18 column （2.1 mm×100 mm， 1.7 μm） with the mobile phase of 0.1% formic acid aqueous solution （A）-acetonitrile （B） for gradient elution （0-15 min， 10%-22%B； 15-20 min， 22%B； 20-25 min， 22%-44%B； 25-35 min， 44%-50%B； 35-40 min， 50%-60%B； 40-55 min， 60%-85%B）， the flow rate was 0.15 mL·min-1， the injection volume was 3 μL and the column temperature was 30 ℃. HRMS was equipped with electrospray ionization （ESI） and scanned in positive and negative ion modes by means of full scan/data dependent secondary scan （Full MS/dd-MS2）. Compound Discoverer 3.0 software combined with mzCloud， mzVault， ChemSpider databases and HRMS database of components in traditional Chinese medicine were used to analyze and identify the collected data by HRMS， based on accurate relative molecular mass， retention time and characteristic ion fragmentation of the compounds， as well as literature information and relevant reference materials. Result:A total of 51 chemical constituents were identified in C. chinensis inflorescence， including 16 alkaloids， 14 flavonoids， 7 phenylpropanoids， 7 organic acids and 7 others. Among them， 10 components ［berberine， palmatine， coptidine， rutin， quercetin， isoquercitrin， chlorogenic acid， cryptochlorogenic acid， D-（-） quinic acid and D-proline］ were unambiguously identified by comparing with reference standards. Conclusion:The established UPLC-Q-Orbitrap HRMS can be used to accurately analyze and identify chemical constituents of C. chinensis inflorescence. A total of 41 chemical constituents are reported from C. chinensis inflorescence for the first time and 6 alkaloids are found from the C. chinensis for the first time. These findings can provide methodological reference and experimental basis for the basic research of quality evaluation and efficacy materials of C. chinensis inflorescence， and lay a foundation for its further development and utilization.
